業績

@article{Okubo2025,

title = {BK Virus Nephropathy Associated With Prolonged Immune Effector Cell-Associated Hematotoxicity Following Anti-CD19 CAR-T-Cell Therapy for Diffuse Large B-Cell Lymphoma in a Nontransplant Patient: A Case Report.},

author = {Naoto Okubo and Hanae Wakabayashi and Daisuke Honda and Yuuki Yoshioka and Tsubasa Shinomiya and Emiko Sakaida and Ryuji Ohashi and Katsuhiko Asanuma},

url = {http://www.ncbi.nlm.nih.gov/pubmed/41342047},

doi = {10.1016/j.xkme.2025.101143},

issn = {2590-0595},

year  = {2025},

date = {2025-12-01},

journal = {Kidney medicine},

volume = {7},

number = {12},

pages = {101143},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Haruna2025,

title = {A Difficult-to-Diagnose Collision Tumor in a Patient With Gastric-Type Duodenal Cancer and Gastric Cancer: A Report of a Rare Case.},

author = {Takahiro Haruna and Ryuji Ohashi and Junji Ueda and Daigo Yoshimori and Hironobu Shioda and Hiroshi Yoshida},

url = {http://www.ncbi.nlm.nih.gov/pubmed/41098299},

doi = {10.7759/cureus.92278},

issn = {2168-8184},

year  = {2025},

date = {2025-09-01},

journal = {Cureus},

volume = {17},

number = {9},

pages = {e92278},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kaseda2025,

title = {Successful treatment of MPO-ANCA positive crescentic IgA nephropathy/IgA vasculitis with nephritis potentially triggered by a COVID-19 vaccine in a young adult female using corticosteroids, rituximab, and avacopan.},

author = {Ken Kaseda and Ryou Terakawa and Rena Matsui and Minoru Yasukawa and Shinichiro Asakawa and Shigeyuki Arai and Osamu Yamazaki and Yoshifuru Tamura and Ryuji Ohashi and Shigeru Shibata and Yoshihide Fujigaki},

url = {http://www.ncbi.nlm.nih.gov/pubmed/40202709},

doi = {10.1007/s13730-025-00991-6},

issn = {2192-4449},

year  = {2025},

date = {2025-08-01},

journal = {CEN case reports},

volume = {14},

number = {4},

pages = {626--632},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Taira2025,

title = {Immunoglobulin A-dominant membranoproliferative glomerulonephritis-like pattern of injury as a possible paraneoplastic nephropathy in a breast cancer patient.},

author = {Shuntaro Taira and Mika Kawagoe and Hitoshi Anzai and Minoru Yasukawa and Shinichiro Asakawa and Shigeyuki Arai and Osamu Yamazaki and Yoshifuru Tamura and Yasutoshi Oshima and Satoe Numakura and Ryuji Ohashi and Shigeru Shibata and Yoshihide Fujigaki},

url = {http://www.ncbi.nlm.nih.gov/pubmed/39365417},

doi = {10.1007/s13730-024-00936-5},

issn = {2192-4449},

year  = {2025},

date = {2025-04-01},

journal = {CEN case reports},

volume = {14},

number = {2},

pages = {217--223},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsui2025,

title = {Coexistence of Sjögren's Syndrome-associated Interstitial Nephritis and Hypokalemic Nephropathy in a Patient with Distal Renal Tubular Acidosis.},

author = {Rena Matsui and Maika Gojo and Kohei Odajima and Shinichiro Asakawa and Shigeyuki Arai and Osamu Yamazaki and Yoshifuru Tamura and Satoe Numakura and Yasutoshi Oshima and Ryuji Ohashi and Shigeru Shibata and Yoshihide Fujigaki},

url = {http://www.ncbi.nlm.nih.gov/pubmed/39198164},

doi = {10.2169/internalmedicine.4152-24},

issn = {1349-7235},

year  = {2025},

date = {2025-04-01},

journal = {Internal medicine (Tokyo, Japan)},

volume = {64},

number = {7},

pages = {1067--1074},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yoshikawa2024,

title = {Fanconi syndrome and renal tubular necrosis in patients following ingestion of potentially contaminated red yeast rice supplement: Two case reports.},

author = {Yoshiyuki Yoshikawa and Hitoshi Anzai and Kohei Odajima and Shinichiro Asakawa and Shigeyuki Arai and Osamu Yamazaki and Yoshifuru Tamura and Ryuji Ohashi and Shigeru Shibata and Yoshihide Fujigaki},

url = {http://www.ncbi.nlm.nih.gov/pubmed/39256890},

doi = {10.14814/phy2.70049},

issn = {2051-817X},

year  = {2024},

date = {2024-09-01},

journal = {Physiological reports},

volume = {12},

number = {17},

pages = {e70049},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yamazaki2024,

title = {Effective Management of Hypertensive Emergencies with Aliskiren Treatment in a Patient before and after Introducing Hemodialysis Secondary to Scleroderma Renal Crisis-like Condition under Corticosteroid Treatment for Sjögren Syndrome-associated Multiple Mononeuropathy.},

author = {Osamu Yamazaki and Masataka Murakawa and Fumika Ochiai and Wataru Fujii and Shinichiro Asakawa and Michito Nagura and Shigeyuki Arai and Yoshifuru Tamura and Ryuji Ohashi and Shigeru Shibata and Yoshihide Fujigaki},

url = {http://www.ncbi.nlm.nih.gov/pubmed/38171869},

doi = {10.2169/internalmedicine.2970-23},

issn = {1349-7235},

year  = {2024},

date = {2024-08-15},

journal = {Internal medicine (Tokyo, Japan)},

volume = {63},

number = {16},

pages = {2301--2306},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Arai2024,

title = {Discontinuing Hemodialysis through Corticosteroid Treatment in a Patient with Cryofibrinogen-associated Glomerulonephritis.},

author = {Shigeyuki Arai and Rena Matsui and Wataru Ono and Shinichiro Asakawa and Osamu Yamazaki and Yoshifuru Tamura and Mika Terasaki and Kanade Hagiwara and Tohru Nakagawa and Ryuji Ohashi and Akira Shimizu and Shigeru Shibata and Yoshihide Fujigaki},

url = {http://www.ncbi.nlm.nih.gov/pubmed/37926528},

doi = {10.2169/internalmedicine.2897-23},

issn = {1349-7235},

year  = {2024},

date = {2024-07-01},

journal = {Internal medicine (Tokyo, Japan)},

volume = {63},

number = {13},

pages = {1899--1905},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yoshioka2024,

title = {A case of nodular cutaneous amyloidosis presenting as refractory leg skin ulcers.},

author = {Ai Yoshioka and Yukako Shintani-Domoto and Ishino Kousuke and Akiharu Kubo and Makoto Kunisada},

url = {http://www.ncbi.nlm.nih.gov/pubmed/38292006},

doi = {10.1111/1346-8138.17117},

issn = {1346-8138},

year  = {2024},

date = {2024-06-01},

journal = {The Journal of dermatology},

volume = {51},

number = {6},

pages = {e211--e213},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Anzai2024,

title = {Slowly Progressive ANCA-associated Glomerulonephritis with Strong Mesangial MPO Deposits Following a Diagnosis of Interstitial Lung Disease.},

author = {Hitoshi Anzai and Yuki Suzuki and Masaki Ueno and Shinichiro Asakawa and Michito Nagura and Shigeyuki Arai and Osamu Yamazaki and Yoshifuru Tamura and Ryuji Ohashi and Shigeru Shibata and Yoshihide Fujigaki},

url = {http://www.ncbi.nlm.nih.gov/pubmed/37722892},

doi = {10.2169/internalmedicine.2512-23},

issn = {1349-7235},

year  = {2024},

date = {2024-05-01},

journal = {Internal medicine (Tokyo, Japan)},

volume = {63},

number = {9},

pages = {1253--1260},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsui2024,

title = {Usefulness of Ultrasonographic Detective Flow Imaging for Detecting Parathyroid Tumors: A Report of Two Cases.},

author = {Mami Matsui and Tomoo Jikuzono and Shoko Kure and Osamu Ishibashi and Haruki Akasu and Iwao Sugitani},

url = {http://www.ncbi.nlm.nih.gov/pubmed/36273907},

doi = {10.1272/jnms.JNMS.2023_90-604},

issn = {1347-3409},

year  = {2024},

date = {2024-01-20},

journal = {Journal of Nippon Medical School = Nippon Ika Daigaku zasshi},

volume = {90},

number = {6},

pages = {460--464},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Hoashi2024,

title = {Japanese Dermatological Association guidelines: Outlines of Japanese clinical guidelines for basal cell carcinoma 2021.},

author = {Toshihiko Hoashi and Masashi Ishikawa and Jiro Uehara and Kohei Oashi and Shintaro Maeda and Junji Kato and Hiroshi Saruta and Ryuji Shichinohe and Ryuji Ohashi and Yoshio Nakamura and Hiroshi Koga and Makoto Sugaya},

url = {http://www.ncbi.nlm.nih.gov/pubmed/38264942},

doi = {10.1111/1346-8138.16944},

issn = {1346-8138},

year  = {2024},

date = {2024-04-01},

journal = {The Journal of dermatology},

volume = {51},

number = {4},

pages = {e90--e105},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Nagaoka2023,

title = {Downhill Varices in the Hypopharynx of a Patient with a Large Thyroid Tumor: A Case Report.},

author = {Ryuta Nagaoka and Marie Saitou and Kiyotaka Nagahama and Ritsuko Okamura and Haruki Akasu and Takehito Igarashi and Kazuhiko Yokoshima and Ryuji Ohashi and Iwao Sugitani},

url = {http://www.ncbi.nlm.nih.gov/pubmed/36273906},

doi = {10.1272/jnms.JNMS.2023_90-601},

issn = {1347-3409},

year  = {2023},

date = {2023-11-07},

journal = {Journal of Nippon Medical School = Nippon Ika Daigaku zasshi},

volume = {90},

number = {5},

pages = {408--413},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ishiguro2023,

title = {A Case of Axillary Fibroadenoma That Grew Rapidly from Axillary Accessory Breast Tissue over 40 Days.},

author = {Akira Ishiguro and Mamiko Tosa and Takashi Sakatani and Ryuji Ohashi and Rei Ogawa},

url = {http://www.ncbi.nlm.nih.gov/pubmed/38025612},

doi = {10.1097/GOX.0000000000005420},

issn = {2169-7574},

year  = {2023},

date = {2023-11-01},

journal = {Plastic and reconstructive surgery. Global open},

volume = {11},

number = {11},

pages = {e5420},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yokota2023,

title = {A Case of Contained Rupture of the Common Iliac Artery with Idiopathic Retroperitoneal Fibrosis: Efficacy of Surgical Treatment and Immunosuppressive Therapy at 2-Year Follow-Up.},

author = {Ryo Yokota and Shun-Ichiro Sakamoto and Tomohiro Murata and Atsushi Hiromoto and Takako Yamaguchi and Kenji Suzuki and Michiko Kobayashi and Shoko Kure and Mitsuhiro Takeno and Yosuke Ishii},

url = {http://www.ncbi.nlm.nih.gov/pubmed/37006861},

doi = {10.3400/avd.cr.22-00120},

issn = {1881-641X},

year  = {2023},

date = {2023-03-25},

journal = {Annals of vascular diseases},

volume = {16},

number = {1},

pages = {95--99},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Okamura2022,

title = {Acute respiratory failure due to Aspergillus niger infection with acute fibrinous and organazing pneumonia: A case report},

author = {Ken Okamura and Rintaro Noro and Kazue Fujita and Shoko Kure and Shinobu Kunugi and Hitoshi Takano and Ryota Miyashita and Takehiro Tozuka and Toru Tanaka and Teppei Sugano and Yumi Sakurai and Ayana Suzuki and Miyuri Suga and Anna Hayashi and Yoshinobu Saito and Kaoru Kubota and Masahiro Seike and Akihiko Gemma},

url = {https://pubmed.ncbi.nlm.nih.gov/35368801/},

doi = {10.1016/J.RMCR.2022.101641},

issn = {2213-0071},

year  = {2022},

date = {2022-01-01},

journal = {Respiratory medicine case reports},

volume = {37},

publisher = {Respir Med Case Rep},

abstract = {A 59-year-old woman complaining of wet cough, hemoptysis, slight fever, anorexia, and malaise was admitted to hospital with suspected lobar pneumonia. She received treatment for myocardial infarction and deep venous thrombosis caused by familial protein C deficiency. Rapid deterioration due to respiratory failure occurred despite intensive care with broad-spectrum antibiotics. At a later date, sputum examination revealed the presence of Aspergillus niger. Based on clinical and autopsy findings, she was diagnosed with acute respiratory failure due to pulmonary aspergillosis with acute fibrinous and organizing pneumonia. This is the first reported case of pulmonary aspergillosis with acute fibrinous and organizing pneumonia complicated by calcium oxalate resulting from Aspergillus niger infection, leading to severe inflammation and tissue injury in the lungs.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jikuzono2022d,

title = {Clinical Utility of Fine-Needle Aspiration Cytology for Adenoid Cystic Carcinoma of the Trachea with Thyroid Invasion: A Case Report.},

author = {Tomoo Jikuzono and Shigekazu Suzuki and Osamu Ishibashi and Shoko Kure and Atsuko Sakanushi and Munenaga Nakamizo and Masashi Kawamoto and Ryuji Ohashi and Tetsu Yamada and Iwao Sugitani},

url = {http://www.ncbi.nlm.nih.gov/pubmed/33867428},

doi = {10.1272/jnms.JNMS.2022_89-207},

issn = {1347-3409},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Nippon Medical School = Nippon Ika Daigaku zasshi},

volume = {89},

issue = {4},

pages = {460-465},

publisher = {Medical Association of Nippon Medical School},

abstract = {BACKGROUND Adenoid cystic carcinoma of the trachea (ACCT) is a rare cancer; ACCT with thyroid invasion is particularly rare. We first suspected anaplastic thyroid carcinoma (ATC) but diagnosed ACC after performing fine-needle aspiration cytology (FNAC). Tracheal origin was confirmed postoperatively. CASE DESCRIPTION A 77-year-old woman presented to our hospital with acute inspiratory dyspnea requiring emergency tracheotomy. Physical examination revealed swelling of the right anterior neck and a hard, immobile mass. Computed tomography (CT) and ultrasonography (US) showed tumor extension to the right thyroid lobe and between the first and third tracheal rings, which caused severe stenosis of the lumen. We performed FNAC. Clinical findings were highly suggestive of ACCT with thyroid invasion. She underwent total laryngectomy, cervical esophagectomy, and thyroidectomy with bilateral selective neck dissection at another hospital. The tumor was located in the right posterior wall of the trachea and extended into the right thyroid gland. Pathological examination showed infiltrative carcinomatous proliferation with tubular and cribriform patterns. The tumor was classified as pT4N1. A definite diagnosis was made after histopathological analysis of the surgical specimen confirmed ACCT. The tumor was positive for FABP7, a putative prognostic marker of ACC, and metastasized to the lungs 3 years after surgery. CONCLUSIONS ACCT with thyroid invasion is an extremely rare malignant neoplasm. FNAC was useful for differentiating ACCT from other diagnoses and enabled appropriate surgical treatment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tonooka2022,

title = {Current Trends in Anti-Cancer Molecular Targeted Therapies: Renal Complications and Their Histological Features},

author = {Akiko Tonooka and Ryuji Ohashi},

doi = {10.1272/jnms.JNMS.2022_89-221},

issn = {13473409},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Nippon Medical School},

volume = {89},

number = {2},

pages = {128--138},

publisher = {Medical Association of Nippon Medical School},

abstract = {Among recent advances in cancer treatment, the emergence of novel drugs targeting specific molecules has considerably modulated therapeutic strategies. Despite the efficacy of these agents, renal complications that are distinct from those of conventional chemotherapeutic drugs have been reported. Targeted therapy drugs include monoclonal antibodies and small-molecule agents. Bevacizumab is a monoclonal antibody that targets vascular endothelial growth factor (VEGF) and blocks tumor angiogenesis. This anti-angiogenic effect causes endothelial injury, resulting in “thrombotic microangiopathy-like lesions” confined to the glomerulus. Segmental hyalinosis of the glomerular tuft is also observed. Small molecular agents, including tyrosine kinase inhibitors (TKIs) such as pazopanib, can cause endothelial injury and podocytopathy by blocking VEGF receptors and their downstream signaling. Minimal change nephrotic syndrome and focal segmental glomerulosclerosis are associated with TKI-induced renal com-plications. Immune checkpoint inhibitors (ICIs) such as PD-1, CTLA-4, and PD-L1 modulate immune checkpoints and are a novel form of immunotherapy against cancer. Owing to their unique function, ICIs cause inflammatory side effects referred to as immune-related adverse events (irAEs). irAEs in the kidney include acute tubulointerstitial nephritis and tubulitis, occasionally accompanied by granuloma formation. Vasculitis, thrombotic microangiopathy, and glomerulonephritis have also been reported. Renal toxicity associated with other molecular drugs, such as protease inhibitors and mammalian target of rapamycin inhibitors, has also been documented. In this article, we review the clinicohistopathological aspects of renal complications associated with molecular targeted therapies and focus on anti-VEGF agents and immune checkpoint inhibitors from a pathological perspective.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kure2021,

title = {Breast Cancer Detection from a Urine Sample by Dog Sniffing: A Preliminary Study for the Development of a New Screening Device, and a Literature Review},

author = {Shoko Kure and Shinya Iida and Marina Yamada and Hiroyuki Takei and Naoyuki Yamashita and Yuji Sato and Masao Miyashita},

url = {https://www.mdpi.com/2079-7737/10/6/517},

doi = {10.3390/biology10060517},

issn = {2079-7737},

year  = {2021},

date = {2021-06-01},

journal = {Biology},

volume = {10},

number = {6},

pages = {517},

abstract = {Background: Breast cancer is a leading cause of cancer death worldwide. Several studies have demonstrated that dogs can sniff and detect cancer in the breath or urine sample of a patient. This study aims to assess whether the urine sample can be used for breast cancer screening by its fingerprints of volatile organic compounds using a single trained sniffer dog. This is a preliminary study for developing the “electronic nose” for cancer screening. Methods: A nine-year-old female Labrador Retriever was trained to identify cancer from urine samples of breast cancer patients. Urine samples from patients histologically diagnosed with primary breast cancer, those with non-breast malignant diseases, and healthy volunteers were obtained, and a double-blind test was performed. Total of 40 patients with breast cancer, 142 patients with non-breast malignant diseases, and 18 healthy volunteers were enrolled, and their urine samples were collected. Results: In 40 times out of 40 runs of a double-blind test, the trained dog could correctly identify urine samples of breast cancer patients. Sensitivity and specificity of this breast cancer detection method using dog sniffing were both 100%. Conclusions: The trained dog in this study could accurately detect breast cancer from urine samples of breast cancer patients. These results indicate the feasibility of a method to detect breast cancer from urine samples using dog sniffing in the diagnosis of breast cancer. Although the methodological standardization is still an issue to be discussed, the current result warrants further study for developing a new breast cancer screening method based on volatile organic compounds in urine samples.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kaneya2021,

title = {Inhibitor for protein disulfide‑isomerase family A member 3 enhances the antiproliferative effect of inhibitor for mechanistic target of rapamycin in liver cancer: An in vitro study on combination treatment with everolimus and 16F16},

author = {Yohei Kaneya and Hideyuki Takata and Ryuichi Wada and Shoko Kure and Kousuke Ishino and Mitsuhiro Kudo and Ryota Kondo and Nobuhiko Taniai and Ryuji Ohashi and Hiroshi Yoshida and Zenya Naito},

doi = {10.3892/ol.2020.12289},

issn = {17921082},

year  = {2021},

date = {2021-01-01},

journal = {Oncology Letters},

volume = {21},

number = {1},

pages = {1--8},

abstract = {mTOR is involved in the proliferation of liver cancer. However, the clinical benefit of treatment with mTOR inhibitors for liver cancer is controversial. Protein disulfide isomerase A member 3 (PDIA3) is a chaperone protein, and it supports the assembly of mTOR complex 1 (mTORC1) and stabilizes signaling. Inhibition of PDIA3 function by a small molecule known as 16F16 may destabilize mTORC1 and enhance the effect of the mTOR inhibitor everolimus (Ev). The aim of the present study was to elucidate the usefulness of combination treatment with Ev and 16F16 in liver cancer using cultured Li‑7 and HuH‑6 cells. The proliferation of cultured cells was examined following treatment with 0.01 µM Ev, 2 µM 16F16 or both. The expression levels and phosphoryla‑ tion of S6 kinase (S6K) and 4E‑binding protein 1 (4E‑BP1) were examined by western blotting. Li‑7 was susceptible to Ev, and proliferation was reduced to 69.5±7.2% by Ev compared with that of untreated cells. Proliferation was reduced to 90.2±10.8% by 16F16 but to 62.3±12.2% by combination treat‑ ment with Ev and 16F16. HuH‑6 cells were resistant to Ev, and proliferation was reduced to 86.7±6.1% by Ev and 86.6±4.8% by 16F16. However, combination treatment suppressed prolif‑ eration to 57.7±4.0%. Phosphorylation of S6K was reduced by Ev in both Li‑7 and HuH‑6 cells. Phosphorylation of 4E‑BP1 was reduced by combination treatment in both Li‑7 and HuH‑6 cells. Immunoprecipitation assays demonstrated that PDIA3 formed a complex with 4E‑BP1 but not with S6K. The small molecule 16F16 increased susceptibility to Ev in cultured liver cancer cells, which are resistant to Ev. The inhibition was associated with reduction of 4E‑BP1 phosphorylation, which formed a complex with PDIA3. Combination treatment with Ev and 16F16 could be a novel therapeutic strategy for liver cancer.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kawamoto2021,

title = {Cytological assessment of desmoplastic malignant pleural mesothelioma in an autopsy case.},

author = {Yoko Kawamoto and Shoko Kure and Hironori Katayama and Kiyoko Kawahara and Kiyoshi Teduka and Shinobu Kunugi and Munehiko Onda and Norio Motoda and Ryuji Ohashi},

url = {http://www.ncbi.nlm.nih.gov/pubmed/34840219},

doi = {10.1272/jnms.JNMS.2022_89-605},

issn = {1347-3409},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Nippon Medical School = Nippon Ika Daigaku zasshi},

publisher = {Medical Association of Nippon Medical School},

abstract = {INTRODUCTION Desmoplastic malignant pleural mesothelioma (DMPM) is a sarcoma type mesothelioma, comprising about 5% of malignant pleural mesotheliomas. Although effusion cytology is commonly used as the primary diagnostic approach for mesothelioma, this may not be useful for DMPM due to its desmoplastic nature and bland cellular atypia. We hereby report a case of DMPM diagnosed through autopsy along with its cytological features that have not been described previously. CASE PRESENTATION A male in his 60s with a history of occupational asbestos exposure was referred to our hospital with right chest pain. Chest computed tomography scan showed right pleural effusion. Thirteen months later, the patient died of respiratory failure. In autopsy, the scrape-imprint smear and the pleural effusions cytology were performed. The scrape-imprint smear samples exhibited spindle cells with mild nuclear atypia and grooves with fibrous stroma. In the pleural effusion cytology, spindle cells having mild nuclear atypia and grooves with loose epithelial connections were observed. Histological examination of the right pleura showed spindle cells proliferating with dense collagen fibers, as seen in cytological samples, thus rendering the diagnosis of DMPM. Diagnosis was confirmed by fluorescence in situ hybridization. CONCLUSION Cytological procedures, such as pleural effusion cytology and scrape-imprinting method, may be useful as an ancillary tool in the diagnosis of rare tumors such as DMPM.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jikuzono2021,

title = {Microarray analysis of ductal carcinoma in situ samples obtained by puncture from surgical resection specimens},

author = {Tomoo Jikuzono and Eriko Manabe and Shoko Kure and Haruki Akasu and Tomoko Ishikawa and Yoko Fujiwara and Masujiro Makita and Osamu Ishibashi},

doi = {10.1186/s13104-021-05760-z},

issn = {17560500},

year  = {2021},

date = {2021-01-01},

journal = {BMC Research Notes},

volume = {14},

issue = {1},

publisher = {BioMed Central Ltd},

abstract = {Objective: The incidence of ductal carcinoma in situ (DCIS) is increasing due to more widespread mammographic screening. DCIS, the earliest form of breast cancer, is non-invasive at the time of detection. If DCIS tissues are left undetected or untreated, it can spread to the surrounding breast tissue. Thus, surgical resection is the standard treatment. Understanding the mechanism underlying the non-invasive property of DCIS could lead to more appropriate medical treatments, including nonsurgical options. Data description: We conducted a microarray-based genome-wide transcriptome analysis using DCIS specimens obtained by puncture from surgical specimens immediately after surgery.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kure2021b,

title = {A prediction model using 2-propanol and 2-butanone in urine distinguishes breast cancer},

author = {Shoko Kure and Sera Satoi and Toshihiko Kitayama and Yuta Nagase and Nobuo Nakano and Marina Yamada and Noboru Uchiyama and Satoshi Miyashita and Shinya Iida and Hiroyuki Takei and Masao Miyashita},

doi = {10.1038/s41598-021-99396-5},

issn = {20452322},

year  = {2021},

date = {2021-01-01},

journal = {Scientific Reports},

volume = {11},

issue = {1},

publisher = {Nature Research},

abstract = {Safe and noninvasive methods for breast cancer screening with improved accuracy are urgently needed. Volatile organic compounds (VOCs) in biological samples such as breath and blood have been investigated as noninvasive novel markers of cancer. We investigated volatile organic compounds in urine to assess their potential for the detection of breast cancer. One hundred and ten women with biopsy-proven breast cancer and 177 healthy volunteers were enrolled. The subjects were divided into two groups: a training set and an external validation set. Urine samples were collected and analyzed by gas chromatography and mass spectrometry. A predictive model was constructed by multivariate analysis, and the sensitivity and specificity of the model were confirmed using both a training set and an external set with reproducibility tests. The training set included 60 breast cancer patients (age 34–88 years, mean 60.3) and 60 healthy controls (age 34–81 years, mean 58.7). The external validation set included 50 breast cancer patients (age 35–85 years, mean 58.8) and 117 healthy controls (age 18–84 years, mean 51.2). One hundred and ninety-one compounds detected in at least 80% of the samples from the training set were used for further analysis. The predictive model that best-detected breast cancer at various clinical stages was constructed using a combination of two of the compounds, 2-propanol and 2-butanone. The sensitivity and specificity in the training set were 93.3% and 83.3%, respectively. Triplicated reproducibility tests were performed by randomly choosing ten samples from each group, and the results showed a matching rate of 100% for the breast cancer patient group and 90% for the healthy control group. Our prediction model using two VOCs is a useful complement to the current diagnostic tools. Further studies inclusive of benign tumors and non-breast malignancies are warranted.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Minnelli2021,

title = {Early Transplant Arteriopathy in Kidney Transplantation},

author = {Carrie Minnelli and Maziar Riazy and Ryuji Ohashi and Jolanta Kowalewska and Nicolae Leca and Behzad Najafian and Kelly D. Smith and Roberto F. Nicosia and Charles E. Alpers and Shreeram Akilesh},

doi = {10.1016/j.transproceed.2021.02.019},

issn = {18732623},

year  = {2021},

date = {2021-01-01},

journal = {Transplantation Proceedings},

volume = {53},

issue = {5},

pages = {1554-1561},

publisher = {Elsevier Inc.},

abstract = {Background: Early dysfunction of renal allografts may be associated with vascular injury, which raises the specter of active rejection processes that require medical intervention. In our practice, we have encountered patients who present with delayed graft function and demonstrate a unique pattern of endothelial cell injury that raises concern for rejection in their biopsy. Therefore, we sought to systematically determine the biopsy characteristics and outcome of these patients. Methods: During a 17-year period at the University of Washington in Seattle, United States, we identified 24 cases of a distinct arterial vasculopathy presenting in the first year posttransplantation. This early transplant arteriopathy (ETA) is characterized by endothelial cell swelling and intimal edema but without the intimal arteritis that defines vascular rejection. Results: Approximately 1% of transplant biopsies during the study period showed ETA, almost all of which were in deceased donor organs (96%), and most presented with delayed graft function (54%) or increased serum creatinine (38%) soon after transplantation (median 13 days; range, 5-139). In this study, 77% of patients were managed expectantly, with only 2 patients (7.6%) subsequently developing acute vascular rejection. Except for 1 patient who died, all patients had functioning allografts at 1 year follow-up. Conclusion: Recognizing ETA and distinguishing it from vascular rejection is important to prevent over-treatment because most patients appear to recover allograft function rapidly with expectant management.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Iwamoto2021,

title = {Neoadjuvant Endocrine Therapy for Operable Breast Cancer: A Retrospective Analysis of Real-World Use},

author = {Miki Iwamoto and Hiroyuki Takei and Jun Ninomiya and Hideki Asakawa and Tomoko Kurita and Keiko Yanagihara and Shinya Iida and Takashi Sakatani and Ryuji Ohashi},

url = {https://pubmed.ncbi.nlm.nih.gov/33692294/},

doi = {10.1272/JNMS.JNMS.2021_88-603},

issn = {1347-3409},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Nippon Medical School = Nippon Ika Daigaku zasshi},

volume = {88},

number = {5},

pages = {448--460},

publisher = {J Nippon Med Sch},

abstract = {Background: A retrospective study of the real-world use of neoadjuvant endocrine therapy (NET) is im-portant for standardizing the role of NET in breast cancer care. Methods: In a consecutive series of women with operable breast cancer who received NET for ≥28 days, associations of NET objectives, NET outcomes, adjuvant chemotherapy use after NET, and survival with clinicopathological factors were examined. Results: NET objectives were reduction in surgical extent in 49 patients, avoidance of surgery in 31, and treatment until scheduled surgery in 8. The mean duration of NET was 349.5 (range, 34-1,923), 869.8 (range, 36-4,859), and 55.8 (range, 39-113) days, respectively, in these cohorts (success rate: 79.6%, 64.5%, and 100%, respectively), and the differences were significant. Among patients in the former two cohorts, progression-free survival was significantly better in patients with stage 0 or I disease, ductal carcinoma in situ or invasive ductal carcinoma, ≥71% estrogen receptor (ER) positivity, and the surgical extent reduction cohort than the other counterparts. Postoperative chemotherapy use was significantly associated with lymph node metastasis, a high Ki67 labeling index, lymphovascular invasion, and a high preoperative endocrine prognostic index at the time of surgery after NET. Better recurrence-free survival after surgery was significantly associated with high ER expression after NET or high progesterone receptor expression before or after NET. Conclusions: NET can help reduce surgical extent or avoid surgery in women with early breast cancer, ductal carcinoma, or high ER expression. NET may also aid in decisions related to postoperative sys-temic therapy to improve survival. (J Nippon Med Sch 2021; 88: 448―460).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kaito2021,

title = {Central Nervous System-related Graft-versus-host Disease after Allogeneic Hematopoietic Stem Cell Transplantation},

author = {Yuta Kaito and Shunsuke Yui and Kazuki Inai and Daishi Onai and Ryosuke Kinoshita and Satoshi Yamanaka and Muneo Okamoto and Ryuichi Wada and Ryuji Ohashi and Koiti Inokuchi and Hiroki Yamaguchi},

url = {https://pubmed.ncbi.nlm.nih.gov/34657908/},

doi = {10.2169/INTERNALMEDICINE.6351-20},

issn = {1349-7235},

year  = {2021},

date = {2021-01-01},

journal = {Internal medicine (Tokyo, Japan)},

volume = {60},

number = {20},

pages = {3299--3304},

publisher = {Intern Med},

abstract = {Allogeneic hemopoietic stem cell transplantation (allo-HSCT) is the only curative therapy for refractory hematological malignancies. However, there are many treatment-related complications, including organ disorders, graft-versus-host disease (GVHD), and infectious diseases. Furthermore, there are many unclear points regarding central nervous system (CNS) complications, and the prognosis in patients with CNS complications is extremely poor. We herein report a 49-year-old woman who developed CNS-GVHD after a second transplantation for therapy-related myelodysplastic syndrome. CNS-GVHD in this case was refractory to all treatments, including steroids, and progressed. We also present a review of the literature about the symptoms, diagnosis, and treatment of CNS-GVHD.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Higuchi2021,

title = {Endosonographic features in patients with non-alcoholic early chronic pancreatitis improved with treatment at one year follow up},

author = {Kazutoshi Higuchi and Seiji Futagami and Hiroshi Yamawaki and Makoto Murakami and Kumiko Kirita and Shuhei Agawa and Go Ikeda and Hiroto Noda and Yasuhiro Kodaka and Nobue Ueki and Keiko Kaneko and Katya Gudis and Ryuji Ohashi and Katsuhiko Iwakiri},

url = {https://pubmed.ncbi.nlm.nih.gov/33536717/},

doi = {10.3164/JCBN.19-130},

issn = {0912-0009},

year  = {2021},

date = {2021-01-01},

journal = {Journal of clinical biochemistry and nutrition},

volume = {68},

number = {1},

pages = {86--94},

publisher = {J Clin Biochem Nutr},

abstract = {Since the prevention of early chronic pancreatitis (ECP) into chronic pancreatitis might be critical for the reduction of pancreatic cancer, we tried to clarify the pathophysiology of ECP patients, focusing on ECP patients without alcoholic chronic pancreatitis. 27 ECP patients without alcoholic chronic pancreatitis and 33 patients with functional dyspepsia with pancreatic enzyme abnormalities (FD-P) were enrolled in this study. Diagnosis of ECP was made when imaging findings showed the presence of more than 2 out of 7 endoscopic ultrasound features. Duodenal degranulated eosinophils and glucagon-like peptide 1 producing cells were estimated by immunostaining. There were no significant differences in characteristics and psychogenic factors between ECP and FD-P patients. Interestingly, endoscopic ultrasound score in ECP patients significantly improved, albeit clinical symptoms in ECP patients showed no improvement at one year follow up. The extent of migration of duodenal degranulated eosinophils in FD-P patients was significantly higher compared to that in ECP patients. The levels of elastase-1 and trypsin in ECP patients with improved endoscopic ultrasound features were significantly reduced by the treatment. Further studies will be needed to clarify whether clinical symptoms and endoscopic ultrasound features in ECP patients without alcoholic chronic pancreatitis were improved in longer follow up study.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ankoh2021,

title = {A rapidly growing small intestinal metastasis from lung cancer},

author = {Katsuyoshi Ankoh and Seiichi Shinji and Takeshi Yamada and Akihisa Matsuda and Ryo Ohta and Hiromichi Sonoda and Masahiro Hotta and Goro Takahashi and Yohei Kaneya and Takuma Iwai and Kohki Takeda and Koji Ueda and Sho Kuriyama and Toshimitsu Miyasaka and Kazuhide Yonaga and Yoshinobu Shioda and Hiroshi Yoshida and Ryuji Ohashi},

url = {https://pubmed.ncbi.nlm.nih.gov/34526470/},

doi = {10.1272/JNMS.JNMS.2022_89-501},

issn = {1347-3409},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Nippon Medical School = Nippon Ika Daigaku zasshi},

publisher = {J Nippon Med Sch},

abstract = {Small intestinal metastasis from lung cancer is a relatively rare occurrence, and often causes intestinal obstruction, gastrointestinal perforation, and/or gastrointestinal bleeding making it is an oncological emergency. Many patients have been reported to undergo emergency surgery owing to the rapid progression of the intestinal metastatic lesion; however, information regarding the changes that occur in such metastases over time remains lacking. Therefore, we analyzed 4 patients who had small intestinal metastases arising from lung cancer, who were treated during a 10-year period (January 2011 to December 2020), and whose tumor diameter changes were recorded. The average tumor volume growth rate was 1.48 (1.31-1.78) -fold, and the median observation period was 22 (4-39) days, showing a rapid increase. Histopathologically, in patients with a high degree of primary tumor atypia, rapid tumor growth caused by intratumoral hemorrhage may occur. This was considered to be one of the factors responsible for the rapid increase in tumor volume.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tomori2021,

title = {Solitary chondrosarcoma of the right ring finger: A case report.},

author = {Yuji Tomori and Norio Motoda and Ryu Tsunoda and Ryuji Ohashi and Yasuyuki Kitagawa and Tokifumi Majima},

url = {http://www.ncbi.nlm.nih.gov/pubmed/34526474},

doi = {10.1272/jnms.JNMS.2022_89-602},

issn = {1347-3409},

year  = {2021},

date = {2021-09-01},

journal = {Journal of Nippon Medical School = Nippon Ika Daigaku zasshi},

publisher = {Medical Association of Nippon Medical School},

abstract = {We present a case of solitary chondrosarcoma arising from the proximal phalanx of the ring finger in an elderly man. The chondrosarcoma developed over a period of 14 years, during which the phalanx became progressively more deformed. Several radiographic investigations were carried out, but the patient declined further suggested diagnostic examinations (computed tomography, magnetic resonance imaging, biopsy). Eventually, the lesion became significantly enlarged, and radiographs showed osteolytic lesions in the phalangeal bone. Ray amputation of the finger was required to establish a wide resection of the chondrosarcoma. Most osteochondral tumors arising from the phalanges are benign tumors such as enchondromas, but primary chondrogenic malignant bone tumors (chondrosarcomas) occasionally occur. Chondrosarcoma of the phalanx is difficult to distinguish from enchondroma of the phalanx, because histological investigations of the two neoplasms often produce similar findings. Even with a combination of clinical, biopsy, and imaging findings, differentiating these neoplasms is still challenging, because the characteristic clinical and radiological features of chondrosarcoma do not appear until it becomes aggressive and starts to cause destructive changes. Once that happens, radical expanded resection of the tumor is essential. Therefore, longstanding enchondroma-like lesions should be actively treated in elderly patients, even if a definite diagnosis of chondrosarcoma cannot be made.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tosaki2021,

title = {Membranous nephropathy with thrombotic microangiopathy-like lesions successfully treated with tocilizumab in a patient with idiopathic multicentric Castleman disease},

author = {Takeshi Tosaki and Masahiro Okabe and Tomo Suzuki and Akihiro Shimizu and Kentaro Koike and Nobuo Tsuboi and Tetsuya Kawamura and Ryuji Ohashi and Shingo Yano and Takashi Yokoo},

doi = {10.1007/s13730-020-00559-6},

issn = {21924449},

year  = {2021},

date = {2021-05-01},

journal = {CEN case reports},

volume = {10},

number = {2},

pages = {265--272},

publisher = {NLM (Medline)},

abstract = {Idiopathic multicentric Castleman disease (iMCD) is a systemic lymphoproliferative disease involving multiple organs, including the kidneys. Membranous nephropathy (MN) has been rarely reported as a complication of iMCD. We herein report the case of a 48-year-old man with a 9-year history of iMCD that was complicated by treatment-resistant nephrotic syndrome due to MN. The first renal biopsy performed at the age of 45 years showed diffuse and global MN with a mild glomerular endothelial injury. He was treated with combined therapy of corticosteroids, immunosuppressants, and tocilizumab, an anti-interleukin-6 (IL-6) receptor monoclonal antibody, which was administered every 2-3 weeks. However, nephrotic syndrome persisted, and renal impairment slowly worsened. Serial biopsy performed at 3 years after the first biopsy confirmed advanced lesions of both MN-related and glomerular endothelial injuries. Modification of the therapeutic strategy to weekly administration of tocilizumab gradually led to the remission of proteinuria, allowing the termination of corticosteroids. Thus, the present case suggests a close link between excessive IL-6 actions and the development of glomerular lesions in iMCD. Successful treatment by strict inhibition of IL-6 actions, in this case, may provide a clue for deciding the therapeutic strategy for severe renal complications associated with iMCD.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Konda2021,

title = {A case of “asymmetrical” Graves’ disease with lateral radioisotope uptake},

author = {Yutaka Konda and Kanshi Minamitani and Tomozumi Takatani and Shoko Kure and Iwao Sugitani},

doi = {10.1111/ped.14575},

issn = {1442200X},

year  = {2021},

date = {2021-01-01},

journal = {Pediatrics International},

volume = {63},

issue = {9},

pages = {1137-1139},

publisher = {John Wiley and Sons Inc},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Nishimoto2021,

title = {Multicentric endocrine mucin-producing sweat gland carcinoma and mucinous carcinoma of the skin: A case report},

author = {Akana Nishimoto and Hiroaki Kuwahara and Ryuji Ohashi and Shin Ansai},

url = {https://pubmed.ncbi.nlm.nih.gov/33047834/},

doi = {10.1111/CUP.13896},

issn = {1600-0560},

year  = {2021},

date = {2021-01-01},

journal = {Journal of cutaneous pathology},

volume = {48},

number = {1},

pages = {165--170},

publisher = {J Cutan Pathol},

abstract = {Endocrine mucin-producing sweat gland carcinoma (EMPSGC) is a rare low-grade sweat gland carcinoma. EMPSGC is thought to be a precursor to mucinous carcinoma of the skin (MCS). Since the first description of EMPSGC in 1997, only a few cases have been reported, and its etiology and mechanisms remain unknown. In this report, we describe a 71-year-old Japanese woman with two isolated EMPSGC and one MCS lesion on her face. She was simultaneously diagnosed with invasive ductal carcinoma of the breast. She had a history of uterine cancer of unknown histopathological diagnosis 24 years previously. The presence of in situ lesions confirmed by myoepithelial cells suggested that the cutaneous lesions were primary tumors. To the best of our knowledge, this is the first case of multiple primary EMPSGC/MCS tumors. Additionally, this might be the first case with multiple primary carcinomas including adnexal cutaneous tumors, breast cancer, and uterine cancer, which may share the common feature of expressing female hormonal receptors. This case indicates that EMPSGC/MCS may be triggered by a hormonal receptor abnormality, perhaps because of genetic defects. A larger number of reports examining this issue may be necessary to further assess our initial observations.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Hayama2021,

title = {Renal Involvement as Rare Acute Tubulointerstitial Nephritis in a Patient with Eosinophilic Disorder Treated with Early Add-on Administration of Mepolizumab},

author = {Yuto Hayama and Fuminori Tomyo and Masaki Ueno and Shinichiro Asakawa and Shigeyuki Arai and Osamu Yamazaki and Yoshifuru Tamura and Makoto Mochizuki and Ryuji Ohashi and Shigeru Shibata and Yoshihide Fujigaki},

url = {https://pubmed.ncbi.nlm.nih.gov/34092738/},

doi = {10.2169/INTERNALMEDICINE.7490-21},

issn = {1349-7235},

year  = {2021},

date = {2021-01-01},

journal = {Internal medicine (Tokyo, Japan)},

volume = {60},

number = {23},

pages = {3759--3764},

publisher = {Intern Med},

abstract = {A 39-year-old man presented with peripheral eosinophilia, pulmonary eosinophilic infiltrate, and renal failure due to acute tubulointerstitial nephritis (TIN). He had experienced childhood asthma and was negative for anti-neutrophil cytoplasmic antibody (ANCA). He was tentatively diagnosed with ANCA-negative eosinophilic granulomatous polyangiitis (EGPA) or idiopathic hypereosinophilic syndrome (HES). Renal involvement of isolated TIN with eosinophil infiltration is rare in EGPA and HES and does not seem to have a good prognosis in the literature. However, his condition improved well with corticosteroids and mepolizumab. The revised classification of EGPA based on the etiology should dictate the proper treatment in suspected EGPA patients with nonsystemic vasculitis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsunaga2021,

title = {An advanced case of gastric histiocytic sarcoma treated with chemotherapy and gastrectomy: a case report and review of literature},

author = {Ryu Matsunaga and Yoshikazu Kanazawa and Kunihiko Matsuno and Daisuke Kakinuma and Taichiro Tokura and Atsushi Marumo and Shunsuke Yui and Fumihiko Ando and Yuka Masuda and Nobutoshi Hagiwara and Tsutomu Nomura and Shunji Kato and Toshiro Yoshiyuki and Yukako Domoto and Takashi Sakatani and Ryuji Ohashi and Koiti Inokuchi and Hiroshi Yoshida},

url = {https://pubmed.ncbi.nlm.nih.gov/34100257/},

doi = {10.1007/S12328-021-01438-Y},

issn = {1865-7265},

year  = {2021},

date = {2021-08-01},

journal = {Clinical journal of gastroenterology},

volume = {14},

number = {4},

pages = {1053--1059},

publisher = {Clin J Gastroenterol},

abstract = {Histiocytic sarcoma is a relatively new disease category and the gastrointestinal origin is sporadic. We report a case of a 74-year-old woman who underwent chemotherapy and proximal gastrectomy for extremely rare, advanced gastric histiocytic sarcoma. The resected specimen was subjected to numerous immunostainings to meet the diagnostic criteria of histiocytic sarcoma and was positive for the histiocyte markers' cluster of differentiation 68 and lysozyme. The markers of Langerhans cells, follicular dendritic cells, and myelocyte were all negative. Six reports of surgical resection of histiocytic sarcoma originating in the stomach exist, including our case. We reviewed the clinical course and the histological and immunohistochemical diagnostic features of surgically resected gastric histiocytic sarcoma.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kure2021c,

title = {Thyroid hürthle cell carcinoma: Clinical, pathological, and molecular features},

author = {Shoko Kure and Ryuji Ohashi},

doi = {10.3390/cancers13010026},

issn = {20726694},

year  = {2021},

date = {2021-01-01},

journal = {Cancers},

volume = {13},

number = {1},

pages = {1--14},

publisher = {MDPI AG},

abstract = {Hürthle cell carcinoma (HCC) represents 3–4% of thyroid carcinoma cases. It is considered to be more aggressive than non-oncocytic thyroid carcinomas. However, due to its rarity, the pathological characteristics and biological behavior of HCC remain to be elucidated. The Hürthle cell is characterized cytologically as a large cell with abundant eosinophilic, granular cytoplasm, and a large hyperchromatic nucleus with a prominent nucleolus. Cytoplasmic granularity is due to the presence of numerous mitochondria. These mitochondria display packed stacking cristae and are arranged in the center. HCC is more often observed in females in their 50–60s. Preoperative diagnosis is challenging, but indicators of malignancy are male, older age, tumor size > 4 cm, a solid nodule with an irregular border, or the presence of psammoma calcifications according to ultrasound. Thyroid lobectomy alone is sufficient treatment for small, unifocal, intrathyroidal carcinomas, or clinically detectable cervical nodal metastases, but total thyroidectomy is recommended for tumors larger than 4 cm. The effectiveness of radioactive iodine is still debated. Molecular changes involve cellular signaling pathways and mitochondria-related DNA. Current knowledge of Hürthle cell carcinoma, including clinical, pathological, and molecular features, with the aim of improving clinical management, is reviewed.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Odajima2021,

title = {Pyuria without Casts and Bilateral Kidney Enlargement Are Probable Hallmarks of Severe Acute Kidney Injury Induced by Acute Pyelonephritis: A Case Report and Literature Review},

author = {Kohei Odajima and Ryo Togashi and Yoshikazu Nemoto and Yuto Hayama and Shinichiro Asakawa and Michito Nagura and Shigeyuki Arai and Osamu Yamazaki and Yoshifuru Tamura and Makoto Mochizuki and Ryuji Ohashi and Shigeru Shibata and Yoshihide Fujigaki},

doi = {10.2169/INTERNALMEDICINE.5721-20},

issn = {13497235},

year  = {2021},

date = {2021-01-01},

journal = {Internal Medicine},

volume = {60},

number = {2},

pages = {293--298},

publisher = {Japanese Society of Internal Medicine},

abstract = {The patient was a 38-year-old man who had experienced nausea and fever for a few days and presented with back pain, oliguria, and pyuria, suggesting acute pyelonephritis (APN). He showed acute kidney injury (AKI) with bilateral kidney enlargement and was using nonsteroidal anti-inflammatory drugs (NSAIDs). AKI-induced by APN was confirmed by kidney biopsy. The AKI was successfully treated with antibiotic therapy. A search of the relevant literature for reports on histopathologically-proven APN-induced severe AKI revealed that the key characteristics were bilateral kidney enlargement with pyuria without casts. Oligoanuria was frequently associated with APN-induced severe AKI, and NSAID use may be a possible risk factor. Prompt antibiotic treatment based on the clinical characteristics of APN-induced AKI can improve the renal outcome.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MINAGAWA2020,

title = {High Expression of p21 as a Potential Therapeutic Target in Ovarian Clear-cell Carcinoma},

author = {YUKI MINAGAWA and KOUSUKE ISHINO and RYUICHI WADA and MITSUHIRO KUDO and ZENYA NAITO and TOSHIYUKI TAKESHITA and RYUJI OHASHI},

url = {http://ar.iiarjournals.org/lookup/doi/10.21873/anticanres.14576},

doi = {10.21873/anticanres.14576},

issn = {0250-7005},

year  = {2020},

date = {2020-10-01},

journal = {Anticancer Research},

volume = {40},

number = {10},

pages = {5631--5639},

abstract = {Background/Aim: DNA damage response (DDR), wherein p21 is a cell fate determinant, is a potential cancer therapeutic target. Molecular expression during DDR was explored in ovarian clear-cell carcinoma (CCC). Materials and Methods: CHK1, CHK2, TP53 and p21 expression in DDR was examined using immunostaining in surgical sections of CCC (n=22). Molecular alterations in two types of CCC cell lines, JHOC-5 and JHOC-9, were investigated using western blot analysis. Results: Expression of DDR-associated molecules was noted in most patients. While high p21 expression was found in half of the patients, the remaining patients exhibited low p21 expression. Treatment with UC2288, a p21 inhibitor, attenuated proliferation of both cell lines, more prominently in JHOC-9, resulting in reduced viability and subsequent apoptosis. Conclusion: p21 Inhibitor induced cell death in cells with high p21 expression, suggesting that p21 suppression can be a therapeutic strategy to treat patients with CCC.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tsunoda2020,

title = {Expression level of long noncoding RNA H19 of normotensive placentas in late pregnancy relates to the fetal growth restriction},

author = {Youhei Tsunoda and Mitsuhiro Kudo and Ryuichi Wada and Kousuke Ishino and Shoko Kure and Takashi Sakatani and Toshiyuki Takeshita and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/32323427/},

doi = {10.1111/jog.14260},

issn = {14470756},

year  = {2020},

date = {2020-07-01},

journal = {Journal of Obstetrics and Gynaecology Research},

volume = {46},

number = {7},

publisher = {Blackwell Publishing},

abstract = {Aim: Infants with fetal growth restriction (FGR) are at an increased risk of perinatal morbidity and mortality. The long noncoding RNA H19 gene is expressed abundantly in placental villi and recent studies suggest that it regulates FGR. However, the role of H19 in the FGR placenta remains unclear. This study aimed to clarify the relationship between H19 expression and FGR using normotensive placentas after 34 weeks of gestation. Methods: Formalin-fixed paraffin-embedded tissues from human placentas collected from pregnancies resulting in small for gestational age (SGA) and appropriate for gestational age (AGA) newborns were used. The histopathological features of placenta tissues, such as villous stromal fibrosis, the numbers of terminal villi, villous vessels and cytotrophoblasts were analyzed using hematoxylin and eosin, Masson's trichrome staining and immunostaining. The localization and expression of H19 in the placentas were demonstrated by in situ hybridization and reverse transcription-quantitative polymerase chain reaction (RT-qPCR), respectively. Moreover, the expression levels of H19-regulated molecules such as IGF2 and decorin (DCN) were measured by RT-qPCR. Results: Histopathological features of the placental villous were not different between placentas associated with SGA and AGA. H19 localized to the villous stroma, endothelial cells and cytotrophoblasts. Moreover, the expression level of H19 in SGA placentas was significantly lower than that in AGA placentas. The expression levels of IGF2 and DCN in SGA placentas tended to be lower than those in AGA placentas similarly to H19. Conclusion: This study highlights the potential importance of regulatory events mediated by H19 in SGA placentas without histopathological abnormalities in late pregnancy.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ishida2020,

title = {Possibility of Local Allergic Rhinitis in Japan},

author = {Mariko Ishida and Shoji Matsune and Nozomu Wakayama and Ryuji Ohashi and Kimihiro Okubo},

url = {https://pubmed.ncbi.nlm.nih.gov/31409100/ https://pubmed.ncbi.nlm.nih.gov/31409100/?from_single_result=Ohashi+R+and+Ishida},

doi = {10.1177/1945892419868441},

issn = {19458932},

year  = {2020},

date = {2020-01-01},

journal = {American Journal of Rhinology and Allergy},

volume = {34},

number = {1},

pages = {26--34},

publisher = {SAGE Publications Inc.},

abstract = {Background: The concept of local allergic rhinitis (LAR) has been advocated recently. Allergic rhinitis in Japan is characterized by house dust mites (HDMs) and Japanese cedar pollen (JCP). To investigate LAR in Japan, total IgE and antigen-specific IgE (sIgE) were measured in inferior turbinate mucosa and their relationships with skin test (ST) and nasal allergen provocation test (NAPT) and as well as serum IgE levels were examined. Methods: Subjects were 50 rhinosinusitis patients for surgery. ST was performed and serum total IgE and sIgE levels were measured preoperatively. Patients with class-0 serum anti-HDM or anti-JCP sIgE levels were subjected to NAPT with HDM or JCP, respectively, or both. In all patients, inferior turbinate mucosa was weighed and mashed, and total IgE and sIgE levels were then measured as local mucosal date per gram and per milligram. Because there is no clinical consensus how to evaluate nasal sIgE yet, both positive NAPT and detectable sIgE in obtained nasal mucosa were adopted as the diagnostic criteria of LAR in order to strictly elucidate the possibility of presence of LAR in Japan. Results: JCP LAR was definitely diagnosed in 2 of 14 patients (14.3%) and HDM LAR in 5 of 21 (23.8%) in cases with rhinosinusitis symptoms in the absence of positive ST nor serum sIgE. Conclusion: The present results positively support LAR by HDM or JCP being present in Japan.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Uchimura2020,

title = {Adipose tissue-derived stem cells suppress coronary arteritis of Kawasaki disease in vivo},

author = {Ryoichi Uchimura and Takahiro Ueda and Ryuji Fukazawa and Jun Hayakawa and Ryuji Ohashi and Noriko Nagi-Miura and Naohito Ohno and Makoto Migita and Yasuhiko Itoh},

url = {https://pubmed.ncbi.nlm.nih.gov/31758839/ https://pubmed.ncbi.nlm.nih.gov/31758839/?from_single_result=Ohashi+R+and+Uchimura},

doi = {10.1111/ped.14062},

issn = {1442200X},

year  = {2020},

date = {2020-01-01},

journal = {Pediatrics International},

volume = {62},

number = {1},

pages = {14--21},

publisher = {Blackwell Publishing},

abstract = {Background: Kawasaki disease (KD) is a systemic inflammatory disease resulting in an acute febrile syndrome commonly affecting children younger than 5 years. Coronary arteritis in KD is occasionally non-responsive to several treatments. Recently, adipose tissue-derived stem cells (ADSCs) have been shown to have anti-inflammatory, immunosuppressive, and tissue-repair characteristics and are considered a useful treatment for inflammatory disease. The present study aimed to elucidate whether the administration of ADSCs can suppress KD-associated vasculitis in vivo. Methods: Candida albicans water-soluble fraction is often used to model KD via the induction of severe coronary arteritis. Kawasaki disease model mice were intravenously administered ADSCs and phosphate-buffered saline (PBS). On day 29, the mice were sacrificed and hearts from mice in each group were dissected. This was followed by serum collection. Cardiac tissue sections were subjected to histopathological examination to evaluate the inflammatory area. The levels of pro-inflammatory cytokines in the serum were analyzed at days 15 and 29. The survival rates of both groups were compared. Results: The mean inflammatory area in coronary arteritis was significantly lower in the ADSC group compared to the PBS group (P textless 0.01). Furthermore, the levels of pro-inflammatory cytokines, such as IL-1$beta$, IL-12, IL-17, RANTES, INF-$gamma$, and TNF-$alpha$, in the ADSC group were significantly lower than those in the PBS group. Moreover, the ADSC group had a significantly higher survival rate than the PBS group. Conclusions: These findings highlight that ADSCs have anti-inflammatory and immune regulatory functions that could provide novel cell-based therapeutic strategies for severe KD.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kuwabara2020,

title = {Unique Clinical and Histological Features of Placental Mesenchymal Dysplasia Complicated by Severe Preeclampsia in the Midtrimester},

author = {Yoshimitsu Kuwabara and Mirei Yonezawa and Yumene Kubota and Tomoko Ichikawa and Ryuji Ohashi and Toshiyuki Takeshita},

url = {https://pubmed.ncbi.nlm.nih.gov/32231865/ https://pubmed.ncbi.nlm.nih.gov/32231865/?from_single_result=Kuwabara+R%2C+Yonezawa+M%2C+Ichikawa+T%2C+Ohashi+R%2C+Takeshita+T},

doi = {10.1055/s-0040-1709186},

issn = {21577005},

year  = {2020},

date = {2020-01-01},

journal = {AJP Reports},

volume = {10},

number = {1},

pages = {E113--E117},

publisher = {Thieme Medical Publishers, Inc.},

abstract = {Detailed clinical and pathological features of placental mesenchymal dysplasia (PMD) complicated by preeclampsia (PE) are unknown. A 39-year-old nulliparous woman was referred at 19 weeks gestation. Ultrasound examination identified a fetus with severe growth restriction (-2.8 SD) and a markedly thickened placenta with many cystic structures suggestive of PMD. At 21 weeks, the patient developed severe hypertension (180/95 mm Hg) with marked proteinuria and an elevated serum soluble fms-like tyrosine-kinase-1 (sFlt-1) level (47,000 pg/L). The pregnancy was terminated to protect maternal health. Placental weight was 450 g and no histopathological findings of either proliferation or dysplasia were observed in the trophoblast. Villous chromosome examination revealed a 46XX karyotype, consistent with the diagnosis of PMD. In addition to the pathological findings of vascular endothelial dysfunction characteristic of the placenta in PE cases, enhanced expression of sFlt-1 in the syncytiotrophoblast of the enlarged villi was confirmed by immunohistochemistry as a novel finding in this condition. Monitoring of the serum sFlt-1 value is suggested to be a useful predictor of the pathological change associated with extremely early severe PE in PMD cases.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2020,

title = {Solid variant of papillary thyroid carcinoma: an under-recognized entity},

author = {Ryuji Ohashi},

url = {https://pubmed.ncbi.nlm.nih.gov/31983699/ https://pubmed.ncbi.nlm.nih.gov/31983699/?from_single_result=Solid+variant+of+papillary+thyroid+carcinoma%3A+an+under-recognized+entity. https://www.jstage.jst.go.jp/article/endocrj/67/3/67_EJ19-0414/_article},

doi = {10.1507/endocrj.EJ19-0414},

issn = {0918-8959},

year  = {2020},

date = {2020-01-01},

journal = {Endocrine Journal},

volume = {67},

number = {3},

pages = {241--248},

publisher = {Japan Endocrine Society},

abstract = {Solid variant of papillary thyroid carcinoma (SVPTC) is a rare morphological variant of papillary thyroid carcinoma (PTC). SVPTC is histologically characterized by predominant solid, trabecular and insular nests of tumor cells while cytological features of PTC such as nuclear grooves and nuclear inclusions are preserved. In fine needle aspiration cytology smears, tumor cells of SVPTC may be presented in cohesive, syncytial or trabecular clusters accompanied by some discohesiveness in the absence of necrosis. Although SVPTC and poorly differentiated thyroid carcinoma (PDTC) share similar histological findings of solid nests, SVPTC can be differentiated from PDTC in the lack of tumor necrosis, severe nuclear atypia, and a higher mitotic index. Immunohistochemical expression of CK19 and HBME-1, common markers of PTC, is decreased in solid nests of SVPTC. In pediatric patients exposed to radiation after the Chernobyl nuclear accident, there was a higher prevalence of SVPTC with RET/PTC3 type rearrangement. BRAF mutations are also reported in a small number of adult patients with SVPTC without any prior radiation exposure. Patients with SVPTC may have a slightly higher incidence of metastasis and recurrence of the tumor compared to conventional PTC, although overall survival rate is comparable. In this article, the current knowledge of SVPTC will be reviewed and discussed with an emphasis on the histopathological feature.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2019a,

title = {M1 macrophage is the predominant phenotype in coronary artery lesions following Kawasaki disease},

author = {Ryuji Ohashi and Ryuji Fukazawa and Akira Shimizu and Shunichi Ogawa and Masami Ochi and Takashi Nitta and Yasuhiko Itoh},

url = {https://pubmed.ncbi.nlm.nih.gov/31621532/},

doi = {10.1177/1358863X19878495},

issn = {14770377},

year  = {2019},

date = {2019-12-01},

journal = {Vascular Medicine (United Kingdom)},

volume = {24},

number = {6},

pages = {484--492},

publisher = {SAGE Publications Ltd},

abstract = {Kawasaki disease (KD) is a systemic inflammatory process that affects the medium-sized arteries, causing various cardiovascular complications. However, it is not clear if the vascular sequelae following KD can predispose to the development of atherosclerosis later in life. Our aim was to examine the macrophage phenotypes in the coronary arteries forming giant aneurysms after KD to gain insight into the pathogenesis of vascular lesions in KD. We examined histological sections of the coronary arteries from five patients with KD who underwent coronary bypass grafting procedure as treatment for giant aneurysms and subsequent stenosis. Immunohistochemical expression of M1- and M2-macrophage markers was assessed to determine the macrophage phenotype of KD to compare with that of atherosclerosis in eight adult patients. All the KD specimens showed a mild to moderate degree of intimal thickening consisting of mature fibrous tissue and distortion of elastic fibers, mimicking the histological features of atherosclerosis. The total number of CD68 positive macrophages was higher in atherosclerosis than in KD specimens. Among the CD68 positive macrophages, the proportion of M1 phenotype, detected by CD86 or SOCS3, was higher in KD than in atherosclerosis. In contrast, the proportion of M2 phenotype, detected by CD163 or MRC1, was higher in patients with atherosclerosis. Despite similar histological features, KD and atherosclerosis appear to have a different immunological etiology for progression of the chronic vascular lesions. A further study enrolling a larger number of cases is required to delineate underlying mechanisms of vascular complications in KD.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kure2019,

title = {Relationship between genetic alterations and clinicopathological characteristics of papillary thyroid carcinoma},

author = {Shoko Kure and Ryuichi Wada and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/30788603/},

doi = {10.1007/s00795-019-00217-6},

issn = {18601499},

year  = {2019},

date = {2019-12-01},

booktitle = {Medical Molecular Morphology},

journal = {Medical Molecular Morphology},

volume = {52},

number = {4},

pages = {181--186},

publisher = {Springer},

abstract = {Papillary thyroid carcinoma (PTC) is characterized by proliferation of follicular cells with distinctive nuclear features such as ground glass appearance, nuclear groove and pseudoinclusion. From the proliferation pattern, PTC is divided into several histological subtypes; conventional histology is classified as papillary type, and there are also follicular and solid variants. PTC is heterogeneous in genetic alterations. PTC with BRAF mutation presents a histology of conventional PTC, and follows an aggressive clinical course. Most cases of PTC with RAS mutation show a follicular variant, and prognosis is favorable. RET/PTC1 is observed sporadically and in young cases, and prognosis is favorable. RET/PTC3 is associated with radiation exposure, and the solid variant is frequent. ETV6-NTRK3 may be associated with radiation exposure, and the clinical course is aggressive. Mutation in the telomerase reverse transcriptase promoter is observed in PTC cases involving elderly male patients. Tumor size is large, associated with distant metastasis and advanced stage. This mutation is found concomitantly with BRAF mutation, and the clinical course is aggressive. Genetic alterations form subsets of PTC with distinct clinicopathological features. Careful assessment of clinicopathological features is considered useful in predicting clinical course and when planning treatment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Hotta2019,

title = {Farnesoid X receptor induces cell death and sensitizes to TRAIL-induced inhibition of growth in colorectal cancer cells through the up-regulation of death receptor 5},

author = {Masahiro Hotta and Takashi Sakatani and Kousuke Ishino and Ryuichi Wada and Mitsuhiro Kudo and Yasuyuki Yokoyama and Takeshi Yamada and Hiroshi Yoshida and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/31561852/},

doi = {10.1016/j.bbrc.2019.09.033},

issn = {10902104},

year  = {2019},

date = {2019-11-01},

journal = {Biochemical and Biophysical Research Communications},

volume = {519},

number = {4},

pages = {824--831},

publisher = {Elsevier B.V.},

abstract = {Farnesoid X receptor (FXR) exhibits critical anti-cancer functions in several types of cancer, including colorectal cancer, in vitro and in vivo. However, the underlying mechanism remains unclear. We evaluated pharmacological activation of FXR with the synthetic agonist GW4064 using comprehensive proteomic analysis in colorectal cancer cell lines (HCT116, SW480, and DLD1). Among the commonly detected proteins in all three cell lines, death receptor 5 (DR5) was the most up-regulated protein, and key autophagy-related proteins, such as microtubule-associated protein 1 light chain 3 alpha/beta (MLP3A/3B) and p62 sequestosome-1 (SQSTM), were also differentially expressed. Western blot analysis showed that GW4064 stimulation induced activation of the extrinsic death signaling pathway in all cell lines and induced activation of the intrinsic death signaling pathway in DLD1 cells. Western blotting showed that DR5 up-regulation was associated with inhibition of autophagic activity. These results suggest that FXR activation induced DR5 up-regulation through inhibition of autophagic activity and the DR5-related death signaling pathway. In addition, DR5 selective ligand, also known as TRAIL, has been widely used for anti-cancer treatment in several clinical trials. Co-treatment of TRAIL with GW4064 synergistically inhibited colorectal cancer cell proliferation as compared with single treatments. To the best of our knowledge, our results provide novel insights into FXR function in cancer cell lines. These findings may contribute to a new therapeutic strategy for colorectal cancer.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Shimoda2019,

title = {Expression of protein disulfide isomerase A3 and its clinicopathological association in gastric cancer},

author = {Tomohiro Shimoda and Ryuichi Wada and Shoko Kure and Kousuke Ishino and Mitsuhiro Kudo and Ryuji Ohashi and Itsuo Fujita and Eiji Uchida and Hiroshi Yoshida and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/30720117/},

doi = {10.3892/or.2019.6999},

issn = {17912431},

year  = {2019},

date = {2019-04-01},

journal = {Oncology Reports},

volume = {41},

number = {4},

pages = {2265--2272},

publisher = {Spandidos Publications},

abstract = {Protein disulfide isomerase A3 (PDIA3) is a chaperone protein that supports the folding and processing of synthesized proteins. Its expression is associated with the prognosis of laryngeal cancer, hepatocellular carcinoma, diffuse glioma and uterine cervical cancer. In the present study, the expression levels of PDIA3 and its clinicopathological association were examined in 52 cases of gastric cancer (GC). The expression of PDIA3 was examined by immunohistochemistry and scored using a semi-quantitative method. According to the score, GC samples were classified into PDIA3‑High and PDIA3‑Low GC. PDIA3‑High GC samples were predominantly of the intestinal type. Multivariate survival analysis indicated that PDIA3 expression and cancer stage were independent factors. The overall survival of PDIA3‑High GC cases was significantly favorable compared with that of PDIA3‑Low GC cases, and this was more evident in cases at an advanced stage. In GC cell cultures, the PDIA3 and major histocompatibility complex (MHC) class I proteins were expressed in three out of the four assessed cell lines according to western blot analysis. Notably, the expression of MHC class I was increased by the stimulation of interferon $gamma$. Co-immunoprecipitation assays suggested the formation of a PDIA3 and MHC class I complex. The findings suggested that PDIA3 may be involved in the immune response of carcinoma cells. The improved prognosis in PDIA3‑High GC may be accounted for, in part, by sufficient antigen processing and expression of MHC class I, which can be mediated by PDIA3. It was suggested that PDIA3 serves an important role in the pathobiology of GC, and that PDIA3 is a useful marker for the prediction of prognosis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kondo2019,

title = {Downregulation of protein disulfide‑isomerase A3 expression inhibits cell proliferation and induces apoptosis through STAT3 signaling in hepatocellular carcinoma},

author = {Ryota Kondo and Kousuke Ishino and Ryuichi Wada and Hideyuki Takata and Wei‑Xia Peng and Mitsuhiro Kudo and Shoko Kure and Yohei Kaneya and Nobuhiko Taniai and Hiroshi Yoshida and Zenya Naito},

url = {http://www.spandidos-publications.com/10.3892/ijo.2019.4710},

doi = {10.3892/ijo.2019.4710},

issn = {1019-6439},

year  = {2019},

date = {2019-02-01},

journal = {International Journal of Oncology},

volume = {54},

pages = {1409--1421},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yokoyama2019,

title = {In vitro and in vivo studies on the association of long non‑coding RNAs H19 and urothelial cancer associated 1 with the susceptibility to 5‑fluorouracil in rectal cancer},

author = {Yasuyuki Yokoyama and Ryuichi Wada and Kousuke Ishino and Mitsuhiro Kudo and Michihiro Koizumi and Takeshi Yamada and Hiroshi Yoshida and Zenya Naito and Takashi Sakatani},

url = {https://pubmed.ncbi.nlm.nih.gov/31638183/},

doi = {10.3892/ijo.2019.4895},

issn = {17912423},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Oncology},

volume = {55},

number = {6},

pages = {1361--1371},

publisher = {Spandidos Publications},

abstract = {There is no predictive biomarker for response to 5‑fluorouracil (5FU)‑based neoadjuvant chemotherapy (NAC) in rectal cancer. In the present study, we examined potential long non‑coding RNAs (lncRNAs) linked to the susceptibility to 5FU in cultured colorectal cancer cells, and in biopsy and resected tissues of 31 human rectal cancer cases treated with NAC. Candidate lncRNAs for the prediction of susceptibility to 5FU were investigated by comprehensive analysis of expression profiles of 84 lncRNAs in cultured cells using PCR array. Bioinformatic analysis identified H19 and urothelial cancer associated 1 (UCA1) as candidate biomarkers for 5FU susceptibility. Quantitative PCR of H19 and UCA1 in cultures of colorectal cancer cells demonstrated the notable variation in expression. The ratios of changes of H19 and UCA1 expression in response to 5FU were low in cells resistant to 5FU, whereas ratios were high in cells susceptible to 5FU. In 5FU‑susceptible cells, cell proliferation was inhibited by 5FU. Upregulation of H19 and UCA1 were associated with the reduction in target molecule expression, including retinoblastoma and p27kip1. In 31 cases of rectal cancer, H19 and UCA1 expression levels in biopsy and resected tissue were comparable. The ratios of H19 and UCA1 expression in resected tissue compared with biopsy samples were low in 17 cases, whereas the ratios were high in 14 cases; 11 of the 17 cases (65%) with low ratios exhibited poor response to NAC, whereas 4 of the 14 cases (29%) with high ratios showed poor response (P=0.045). The increase in H19 and UCA1 expression may represent the response to impaired cell cycle in cells susceptible to 5FU. Our results indicate that changes in H19 and UCA1 expression may be considered for predicting the susceptibility to 5FU‑based NAC in rectal cancer.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2019,

title = {Editorial comment on the fate of full-house immunofluorescence staining in renal allograft: A case report},

author = {Ryuji Ohashi},

url = {https://pubmed.ncbi.nlm.nih.gov/31535415/ https://pubmed.ncbi.nlm.nih.gov/31535415/?from_single_result=Ohashi+R+and+renal+allograft},

doi = {10.1111/pin.12848},

issn = {14401827},

year  = {2019},

date = {2019-11-01},

booktitle = {Pathology International},

journal = {Pathology International},

volume = {69},

number = {11},

pages = {661},

publisher = {Blackwell Publishing},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yasukawa2019,

title = {A patient with MPO-ANCA-positive IgA nephropathy diagnosed with the clinical onset of macrohematuria},

author = {Minoru Yasukawa and Sachiko Kitagawa and Ryo Togashi and Shinichiro Asakawa and Michito Nagura and Shigeyuki Arai and Osamu Yamazaki and Yoshifuru Tamura and Fukuo Kondo and Ryuji Ohashi and Shunya Uchida and Shigeru Shibata and Yoshihide Fujigaki},

url = {https://pubmed.ncbi.nlm.nih.gov/30918194/ https://pubmed.ncbi.nlm.nih.gov/30918194/?from_single_result=A+patient+of+MPO-ANCA-positive+IgA+nephropathy+diagnosed+with+clinical+onset+of+macrohematuria.},

doi = {10.2169/internalmedicine.2475-18},

issn = {13497235},

year  = {2019},

date = {2019-07-01},

journal = {Internal Medicine},

volume = {58},

number = {14},

pages = {2051--2056},

publisher = {Japanese Society of Internal Medicine},

abstract = {A 21-year-old woman presented with renal dysfunction during macrohematuria. A kidney biopsy revealed IgA nephropathy with a small percentage of crescent formation and macrohematuria-associated tubular injury. Macrohematuria-associated acute kidney injury could explain her renal dysfunction. However, she was seropositive for myeloperoxidase (MPO)-anti-neutrophil cytoplasmic antibody (ANCA) and showed fibrin deposition around one arteriole. Corticosteroids and mycophenolate mofetil were administered as for ANCA vasculitis, and the serum creatinine, abnormal urinalysis and MPO-ANCA titer all gradually ameliorated. The presence of extra-glomerular vasculitis, which was probably induced by ANCA, suggested that MPO-ANCA was an exacerbating factor for her prolonged renal dysfunction. This condition has so far only rarely been addressed in ANCA-positive IgA nephropathy.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Motoda2019,

title = {Crystalloid Granuloma of Parotid Gland: A Case Report With Review of the Literature},

author = {Norio Motoda and Munehiko Onda and Munenaga Nakamizo and Ryuji Ohashi and Kimihiro Okubo and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/30051740/},

doi = {10.1177/1066896918791031},

issn = {19402465},

year  = {2019},

date = {2019-04-01},

journal = {International Journal of Surgical Pathology},

volume = {27},

number = {2},

pages = {216--220},

publisher = {SAGE Publications Inc.},

abstract = {Crystalloid granuloma (CG) of salivary gland is an extremely rare inflammatory disease, and only 6 cases have been reported in the English literature. CG is histologically characterized by a granulomatous reaction to amylase crystalloid deposition. A 73-year-old woman presented with a painful left neck mass. Computed tomography depicted a mass located in the lower pole of the left parotid gland, suspicious for a tumoral lesion. Preoperative fine needle aspiration cytology found amylase crystalloid deposition with a few inflammatory cells. Surgical sections of the mass revealed formation of a granuloma containing abundant eosinophilic but glassy and transparent amorphous crystalloids, suggestive of $alpha$-amylase crystalloid. No neoplastic elements were detected. The case was eventually diagnosed with CG in the parotid gland. Our findings suggest that when we identify amylase crystalloids in fine needle aspiration cytology smears from the salivary gland, CG should be considered even if neoplasm is clinically or radiographically suspected.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Motoda2019b,

title = {Crystalloid Granuloma of Parotid Gland: A Case Report With Review of the Literature},

author = {Norio Motoda and Munehiko Onda and Munenaga Nakamizo and Ryuji Ohashi and Kimihiro Okubo and Zenya Naito},

url = {http://www.ncbi.nlm.nih.gov/pubmed/30051740 https://pubmed.ncbi.nlm.nih.gov/30051740/ http://journals.sagepub.com/doi/10.1177/1066896918791031},

doi = {10.1177/1066896918791031},

issn = {1066-8969},

year  = {2019},

date = {2019-04-01},

journal = {International Journal of Surgical Pathology},

volume = {27},

number = {2},

pages = {216--220},

publisher = {SAGE Publications Inc.},

abstract = {Crystalloid granuloma (CG) of salivary gland is an extremely rare inflammatory disease, and only 6 cases have been reported in the English literature. CG is histologically characterized by a granulomatous reaction to amylase crystalloid deposition. A 73-year-old woman presented with a painful left neck mass. Computed tomography depicted a mass located in the lower pole of the left parotid gland, suspicious for a tumoral lesion. Preoperative fine needle aspiration cytology found amylase crystalloid deposition with a few inflammatory cells. Surgical sections of the mass revealed formation of a granuloma containing abundant eosinophilic but glassy and transparent amorphous crystalloids, suggestive of $alpha$-amylase crystalloid. No neoplastic elements were detected. The case was eventually diagnosed with CG in the parotid gland. Our findings suggest that when we identify amylase crystalloids in fine needle aspiration cytology smears from the salivary gland, CG should be considered even if neoplasm is clinically or radiographically suspected.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WX2019,

title = {Superficial Myofibroblastoma in the Vulva Mimicking Aggressive Angiomyxoma: A Case Report and Review of the Literature},

author = {Peng WX and Wada R and Kure S and Fukunaga M and Naito Z},

url = {https://pubmed.ncbi.nlm.nih.gov/31240143/},

doi = {10.1155/2019/1582714},

issn = {2090-6781},

year  = {2019},

date = {2019-01-01},

journal = {Case reports in pathology},

volume = {2019},

publisher = {Case Rep Pathol},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{F2019,

title = {A Case of Rheumatoid Arthritis Presenting with Renal Thrombotic Microangiopathy Probably due to a Combination of Chronic Tacrolimus Arteriolopathy and Severe Hypertension},

author = {Honma F and Fujigaki Y and Nemoto Y and Kikuchi H and Nagura M and Arai S and Ishizawa K and Yamazaki O and Tamura Y and Kondo F and Ohashi R and Uchida S and Shibata S},

url = {https://pubmed.ncbi.nlm.nih.gov/30963011/ https://pubmed.ncbi.nlm.nih.gov/30963011/?from_single_result=A+case+of+rheumatoid+arthritis+presenting+with+renal+thrombotic+microangiopathy+probably+due+to+a+combination+of+chronic+tacrolimus+arteriolopathy+and+severe+hypertension.},

doi = {10.1155/2019/3923190},

issn = {2090-6641},

year  = {2019},

date = {2019-01-01},

journal = {Case reports in nephrology},

volume = {2019},

publisher = {Case Rep Nephrol},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{R2019,

title = {Clinicopathological Implications of Proteinuria after Long-Term Isolated Hematuria due to Thin Basement Membrane Nephropathy and Focal Segmental Glomerulosclerosis},

author = {Togashi R and Nemoto Y and Waki K and Nagura M and Arai S and Tamura Y and Oshima Y and Kondo F and Ohashi R and Uchida S and Shibata S and Fujigaki Y},

url = {https://pubmed.ncbi.nlm.nih.gov/31976098/ https://pubmed.ncbi.nlm.nih.gov/31976098/?from_single_result=Clinicopathological+implications+of+proteinuria+after+long-term+isolated+hematuria+due+to+thin+basement+membrane+nephropathy+and+focal+segmental+glomerulosclerosis.},

doi = {10.1155/2019/1627392},

issn = {2090-6641},

year  = {2019},

date = {2019-01-01},

journal = {Case reports in nephrology},

volume = {2019},

publisher = {Case Rep Nephrol},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kanzaki2019,

title = {Monoclonal Immunoglobulin Deposition Disease and Related Diseases},

author = {Go Kanzaki and Yusuke Okabayashi and Kiyotaka Nagahama and Ryuji Ohashi and Nobuo Tsuboi and Takashi Yokoo and Akira Shimizu},

url = {https://pubmed.ncbi.nlm.nih.gov/30918151/ https://pubmed.ncbi.nlm.nih.gov/30918151/?from_single_result=Kanzaki+G+and+Ohashi https://www.jstage.jst.go.jp/article/jnms/86/1/86_JNMS.2019_86-1/_article},

doi = {10.1272/jnms.JNMS.2019_86-1},

issn = {1345-4676},

year  = {2019},

date = {2019-01-01},

journal = {Journal of Nippon Medical School},

volume = {86},

number = {1},

pages = {2--9},

publisher = {Medical Association of Nippon Medical School},

abstract = {Abnormal proliferation of plasma cells and some monoclonal B cells frequently cause the secretion of monoclonal immunoglobulins or immunoglobulin fragments into the serum, causing monoclonal gammopathy, which leads to various diseases including renal diseases. Therefore, monoclonal gammopathy is frequently associated with kidney diseases, including glomerular and tubulointerstitial diseases. Glomerular disease, with the deposition of monoclonal immunoglobulins or their components, includes monoclonal immunoglobulin deposition disease (MIDD), AL or AH amyloidosis, type I cryoglobulinemia, proliferative glomerulonephritis with monoclonal IgG deposits (PGNMID), immunotactoid glomerulopathy, and fibrillary glomerulonephritis. In addition, tubulointerstitial diseases with the deposition of monoclonal immunoglobulins or their components are constituted by light chain (myeloma) cast nephropathy, light chain associated Fanconi's syndrome (light chain proximal [crystal] tubulopathy), and crystal-storing histiocytosis. In the present review article, we demonstrate the clinicopathological characteristics of MIDD, which is one of the representative diseases of plasma cell dyscrasias, and discuss various renal diseases with the deposition of monoclonal immunoglobulins or their components in glomeruli and the tubulointerstitium. We recommend that these renal diseases are arranged as one disease category, “renal diseases with deposition of monoclonal immunoglobulins or their components”, in order to simplify the understanding of complicated diseases in plasma cell dysplasia.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tomori2018,

title = {Intramuscular Epithelioid Sarcoma Presenting as Extrinsic Flexor Tightness in the Forearm},

author = {Yuji Tomori and Ryuji Ohashi and Takuya Sawaizumi and Zenya Naito and Mitsuhiko Nanno and Shinro Takai},

url = {https://pubmed.ncbi.nlm.nih.gov/29602652/ https://pubmed.ncbi.nlm.nih.gov/29602652/?from_single_result=Intramuscular+epithelioid+sarcoma+presenting+as+extrinsic+flexor+tightness+in+the+forearm%3A+a+case+report.},

doi = {10.1016/j.jhsa.2018.02.020},

issn = {15316564},

year  = {2018},

date = {2018-10-01},

journal = {Journal of Hand Surgery},

volume = {43},

number = {10},

pages = {954.e1--954.e5},

publisher = {W.B. Saunders},

abstract = {Epithelioid sarcoma is an uncommon soft tissue sarcoma involving predominantly the distal extremities of adolescents and young adults. Its rarity makes it difficult to diagnose accurately and treat properly in the early stages. We discuss the delayed diagnosis of a 37-year-old man who presented with extrinsic flexor tightness of the wrist and fingers. We initially thought that the lesion resulted from inflamed soft tissue of the flexor muscles causing contracture. However, histological examination of a biopsy specimen revealed nodular proliferation of epithelioid and spindle cells, which were immunoreactive to epithelial and nonepithelial markers, respectively, leading to the final diagnosis of epithelioid sarcoma.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tomori2018a,

title = {Preiser disease after repeated local glucocorticoid injections: A case report},

author = {Yuji Tomori and Norio Motoda and Ryuji Ohashi and Takuya Sawaizumi and Mitsuhiko Nanno and Shinro Takai},

url = {https://pubmed.ncbi.nlm.nih.gov/30235715/ https://pubmed.ncbi.nlm.nih.gov/30235715/?from_single_result=Local+repeated+glucocorticoid+injection+followed+by+Preiser+disease%3A+A+case+report.},

doi = {10.1097/MD.0000000000012413},

issn = {15365964},

year  = {2018},

date = {2018-09-01},

journal = {Medicine (United States)},

volume = {97},

number = {38},

publisher = {Lippincott Williams and Wilkins},

abstract = {Rationale: Preiser disease or avascular necrosis (AVN) of the scaphoid causes intolerable wrist pain and malalignment of the carpal bones. In previously reported cases, patients have had a history of steroid use for systemic illness such as autoimmune hemolytic anemia, systemic lupus erythematosus, or renal transplantation, or have had other risk factors, such as smoking, alcoholism, or infection. In particular, systemic glucocorticoid therapy has been most commonly associated with the disease. Although there are reports of AVN of the scaphoid induced by systemic glucocorticoids, no prior report has associated AVN of the carpal bones with repeated local injections of glucocorticoids. Patient concerns: We present a case in which it was strongly suspected that AVN of the scaphoid was induced by repeated local glucocorticoid injections. The patient had no history of excessive alcohol use, smoking, or trauma, except for local repeated steroid injections. Diagnoses: Initially, she had diagnosed with de Quervain's disease and was treated by repeated local glucocorticoid injections followed by surgery for de Quervain's disease. Five years after surgery for de Quervain's disease, the patient presented at our hospital with sudden onset of intolerable pain in her right wrist without a history of trauma. In spite of nonsurgical treatment with rest, immobilization, analgesia, and surgery, her wrist pain was not improved. After further repeated local steroid injections in her wrist, radiographs, and magnetic resonance imaging of her wrist showed the AVN of the scaphoid. Interventions: Surgery was performed and the fragmented proximal scaphoid and the entire lunate were resected. Outcomes: The diagnosis was confirmed according to the histopathological examination of the proximal scaphoid bone, which showed the characteristic of AVN of the scaphoid. At follow-up evaluation, radiographs of the right wrist showed no progression of osteoarthritis. The patient had no tenderness or residual pain at the wrist and had no desire to pursue additional surgery. Lessons: We have presented a case with AVN of the scaphoid, which was strongly suspected to be associated with the repeated local steroid injections. Further studies are required to more fully elucidate the association between AVN of the scaphoid and repeated local steroid injections.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Okabe2018,

title = {A case of infection-related glomerulonephritis with massive eosinophilic infiltration},

author = {Masahiro Okabe and Tsuyoshi Takamura and Akiko Tajiri and Nobuo Tsuboi and Masahiro Ishikawa and Makoto Ogura and Ryuji Ohashi and Takashi Oda and Takashi Yokoo},

url = {https://pubmed.ncbi.nlm.nih.gov/29701177/},

doi = {10.5414/CN109415},

issn = {03010430},

year  = {2018},

date = {2018-08-01},

journal = {Clinical Nephrology},

volume = {90},

number = {2},

pages = {142--147},

publisher = {Dustri-Verlag Dr. Karl Feistle},

abstract = {Infection-related glomerulonephritis (IRGN) is rarely complicated with eosinophil infiltration into the glomerulus. Here we report a case of eosinophilic proliferative glomerulonephritis related with infection. A 70-year-old man with respiratory symptoms displayed hypereosinophilia, hypocomplementemia, impaired renal function, and nephrotic syndrome. Renal biopsy revealed endocapillary proliferative glomerulonephritis with immunostaining for immunoglobulin G and complement 3, and subepithelial hump-like electron-dense deposits, thus fulfilling the criteria for IRGN. Immunostaining for the nephritis-associated plasmin receptor (NAPlr) in the glomerulus confirmed the diagnosis of IRGN. Of note, eosinophils infiltrated into the glomerular subendothelial spaces, renal tubules, peritubular capillaries, and the interstitium in the kidney as well as in the alveolar walls and pulmonary arteries in the lung. Corticosteroid therapy rapidly improved hypereosinophilia as well as respiratory symptoms and renal function. Urinary protein exertion was decreased, and serum level of complement and albumin was increased. These findings suggest that eosinophil infiltration might play a prominent role in respiratory and renal disorders. Severe endothelial damage of glomeruli and tubulointerstitial nephritis, caused by eosinophil-rich inflammation, might significantly contribute to exacerbation of renal insufficiency.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Endo2018,

title = {Carcinosarcoma ex pleomorphic adenoma of the submandibular gland in a 64-year-old man: A case report},

author = {Yoko Endo and Ryuji Ohashi and Shunta Inai and Kazuhiko Yokoshima and Munenaga Nakamizo and Akira Shimizu and Kimihiro Okubo and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/29540647/},

doi = {10.1272/jnms.2018_85-8},

issn = {13473409},

year  = {2018},

date = {2018-01-01},

journal = {Journal of Nippon Medical School},

volume = {85},

number = {1},

pages = {51--55},

publisher = {Medical Association of Nippon Medical School},

abstract = {Carcinosarcoma (CS) is a rare tumor, consisting of both carcinomatous and sarcomatous components. In this paper, we present a case of CS arising from a pleomorphic adenoma (PA) of the submandibular gland. A 64-year-old Japanese man presented with a left submandibular mass that had developed for 20 years with complaints of pain for the last 3 months. Magnetic resonance imaging showed a lesion involving the left submandibular gland. The patient underwent total dissection of the left submandibular gland and left cervical lymph nodes. Upon gross examination, the mass appeared completely covered by fibroadipose tissue measuring 46×42×45 mm; sectioning revealed a solid-white nodule with central bleeding and necrosis, invading into the surrounding adipose tissue. Microscopically, the presence of carcinomatous and sarcomatous components in the fibro-myxomatous stroma was detected, suggestive of pre-existing PA. The carcinoma component was diagnostic of salivary adenocarcinoma, not otherwise specified, whereas the sarcomatous component exhibited features of osteosarcoma characterized by formation of osteoid. As the border between the carcinomatous and sarcomatous components was not evident, CS may have occurred via transformation of the carcinoma into sarcoma. Tumor metastasis was detected in the cervical lymph nodes. Immunohistochemically, AE1/AE3 expression was noted in the carcinomatous component, but not in the osteosarcoma component. Both components were diffusely positive for vimentin. Four months after the operation, the patient developed a metastatic CS lesion in the lung, suggesting tumor aggression.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2018a,

title = {Breast carcinoma with osteoclast-like giant cells: A cytological-pathological correlation with a literature review},

author = {Ryuji Ohashi and Ayako Hayama and Miyuki Matsubara and Yasuhiko Watarai and Takashi Sakatani and Zenya Naito and Akira Shimizu},

url = {https://pubmed.ncbi.nlm.nih.gov/29566940/ https://pubmed.ncbi.nlm.nih.gov/29566940/?from_single_result=Breast+carcinoma+with+osteoclast-like+giant+cells%3A+A+cytological-pathological+correlation+with+a+literature+review},

doi = {10.1016/j.anndiagpath.2017.11.003},

issn = {15328198},

year  = {2018},

date = {2018-04-01},

journal = {Annals of Diagnostic Pathology},

volume = {33},

pages = {1--5},

publisher = {W.B. Saunders},

abstract = {Breast carcinoma with osteoclast-like giant cells (OGCs) is a rare disease characterized by the infiltration of OGCs in the tumor; however, cytological aspects of this tumor type remain elusive. We examined the cytological features in fine needle aspiration (FNA) biopsy smears obtained from 5 patients who were histologically diagnosed with breast carcinoma with OGCs. We compared FNA and clinicopathological findings with results from the published literature. Histological assessment of the resected samples showed that all tumors exhibited a histological grade 1 phenotype with a predominant cribriform architecture. Four patients were estrogen receptor positive, and 1 patient showed a triple negative phenotype. All patients survived without tumor recurrence. In the FNA smears, tumor cells were arranged in loosely cohesive clusters, characterized by varying degrees of OGCs infiltration and rare formation of solid tumor nests. Occasionally, 2- or 3-dimensional clusters of tumor cells were found, accompanied by OGCs at the peripheral regions. In all patients, tumor cells were small without severe nuclear atypia. None of the patients showed significant background necrosis. In summary, cytological features of breast carcinoma with OGCs are characterized by loose aggregates of low grade tumor cells, the presence of OGCs, and the absence of necrosis, all of which were consistent with features reported previously. This peculiar form of breast tumors should be included in the differential diagnosis, when physicians encounter FNA findings including low grade ductal carcinoma with the admixture of multinucleated giant cells or OGCs.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2018b,

title = {Osteoclast-like giant cells in invasive breast cancer predominantly possess M2-macrophage phenotype},

author = {Ryuji Ohashi and Keiko Yanagihara and Shigeki Namimatsu and Takashi Sakatani and Hiroyuki Takei and Zenya Naito and Akira Shimizu},

url = {https://pubmed.ncbi.nlm.nih.gov/29129494/ https://pubmed.ncbi.nlm.nih.gov/29129494/?from_single_result=Osteoclast-like+giant+cells+in+invasive+ductal+carcinoma+predominantly+possess+M2-macrophage+phenotype},

doi = {10.1016/j.prp.2017.11.002},

issn = {16180631},

year  = {2018},

date = {2018-02-01},

journal = {Pathology Research and Practice},

volume = {214},

number = {2},

pages = {253--258},

publisher = {Elsevier GmbH},

abstract = {Breast carcinoma with osteoclast-like giant cells (OGCs) is a rare tumor; however, their clinicopathological aspects remain unclear. We described the clinicopathological characteristics of 8 patients with breast carcinoma with OGCs. Immuno-phenotypes of the OGCs were comparatively examined with that of foreign body giant cells (FBGCs) in 4 cases of granulomatous reaction (GR) without cancerous elements. In most cancers, tumors displayed cribriform and tubular growth patterns. Three cases showed moderate to high nuclear grade, while all the other tumors had low nuclear grade. Six patients were estrogen receptor (ER) positive, while triple negative phenotype was identified in 2 patients. During the follow-up period, 1 patient had local recurrence of the tumor, and all the patients remained alive. All OGCs and FBGCs expressed CD68, a pan-macrophage marker. OGCs in all the breast cancers showed moderate to high expression of CD163 — a marker of M2-macrophage with pro-tumoral function — whereas its expression in FBGCs was low to moderate (p = 0.04). CD86 — a marker of M1-macrophage with a tumoricidal activity — was positive in the OGCs of 3 breast cancers, and in the FBGCs of 3 GR cases (p = 0.15). The expression of CD163 was significantly higher than that of CD86 in the OGCs of breast cancer (p textless 0.001), whereas they were comparable in the FBGCs of GR (p = 0.79). In summary, we found that breast carcinoma with OGCs mostly exhibited cribriform and tubular growth pattern, ER positivity, and predominantly possessed the M2-macrophage phenotype. However, the clinical significance of OGCs in breast cancer needs to be elucidated in further studies involving a larger number of cases.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2017a,

title = {IMP3 contributes to poor prognosis of patients with metaplastic breast carcinoma: A clinicopathological study},

author = {Ryuji Ohashi and Maoka Sangen and Shigeki Namimatsu and Hiroyuki Takei and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/29146055/},

doi = {10.1016/j.anndiagpath.2017.05.015},

issn = {15328198},

year  = {2017},

date = {2017-12-01},

journal = {Annals of Diagnostic Pathology},

volume = {31},

pages = {30--35},

publisher = {W.B. Saunders},

abstract = {Metaplastic breast carcinoma (MBC) is a rare type of tumor with heterogenous histological patterns. We investigated the immunohistochemical expression of IMP3, an oncofetal protein, in 31 MBC patients in association with histological subtypes and clinical outcomes. The cohort consisted of spindle cell carcinoma (SPC) (n = 11), squamous cell carcinoma (SCC) (n = 14), matrix-producing carcinoma (MPC) (n = 4), carcinoma with osteocartilaginous elements (COC) (n = 1), and low grade adenosquamous cell carcinoma (ASC) (n = 1). IMP3 expression was identified in 7 cases of SPC (64%) and 6 patients of all the other subtypes (p = 0.051). In comparison between IMP3 high (n = 13) and low (n = 18) groups, a large-sized tumor (≥ 4.0 cm) was identified in 9 IMP3 high patients, and 14 IMP3 low patients had a small-sized tumor (p = 0.01). High Ki67 positivity was detected in all of the IMP3 high patients and in 7 of the IMP3 low patients (p = 0.002). During the follow-up period, 9 IMP3 high patients died, whereas 15 of the 18 IMP3 low patients remained alive (p = 0.004). A univariate analysis revealed that IMP3 expression and tumor size were significantly associated with poor clinical outcomes (p = 0.03 and textless 0.001, respectively). The IMP3 high group was likely to be associated with reduced overall survival compared to the IMP3 low group (p = 0.06). These findings indicate that IMP3 may contribute to the aggressive behavior of MBC, and that this expression could potentially be a prognostic marker of MBC.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsumoto2017a,

title = {Histological analysis of hyalinised keloidal collagen formation in earlobe keloids over time: collagen hyalinisation starts in the perivascular area},

author = {Noriko M Matsumoto and Wei Xia Peng and Masayo Aoki and Satoshi Akaishi and Ryuji Ohashi and Rei Ogawa and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/28467018/ https://pubmed.ncbi.nlm.nih.gov/28467018/?from_single_result=Histological+analysis+of+hyalinized+keloidal+collagen+formation+in+earlobe+keloids+over+time%3A+collagen+hyalinization+starts+in+the+perivascular+area.},

doi = {10.1111/iwj.12763},

issn = {1742481X},

year  = {2017},

date = {2017-12-01},

journal = {International Wound Journal},

volume = {14},

number = {6},

pages = {1088--1093},

publisher = {Blackwell Publishing Ltd},

abstract = {Keloids grow and do not regress. They are characterised histologically by hyalinised keloidal collagen (HKC). HKC amounts vary, and the mechanism by which they form is unclear. To clarify how HKCs form and whether their formation associates with specific clinical features, we studied the histological findings of earlobe keloids and compared them with respective clinical features. A total of 50 earlobe keloids from 43 patients were used for histological analysis of keloid size (mm2), HKC area (mm2) and HKC area ratio (%). As a result, keloid durations ranged from 3 months to textgreater13 years. Early-stage keloids exhibited little HKC and a tendency for the HKCs to locate in perivascular regions. In later-stage keloids, the HKCs were extremely interconnected and formed a thick bitten donut-shaped region. HKC area ratios correlated positively with keloid duration (r2 = 0textperiodcentered58, Ptextless0textperiodcentered05). HKC area ratios and keloid durations did not correlate with keloid sizes. These patterns of HKC formation and growth may explain why local therapies, which effectively remove fibroblasts and accumulated collagen but not HKCs, are ineffective in older keloids. Keloids should be promptly treated after diagnosis, and older keloids with extensive HKCs may require surgical excision followed by radiotherapy.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2017e,

title = {Characterization of a murine model with arteritis induced by Nod1 ligand, FK565: A comparative study with a CAWS-induced model},

author = {Ryuji Ohashi and Ryuji Fukazawa and Makoto Watanabe and Koji Hashimoto and Nobuko Suzuki and Noriko Nagi-Miura and Naohito Ohno and Akira Shimizu and Yasuhiko Itoh},

url = {https://pubmed.ncbi.nlm.nih.gov/28150515/ https://pubmed.ncbi.nlm.nih.gov/28150515/?from_single_result=Characterization+of+a+murine+model+with+arthritis+induced+by+NOD1+ligand%2C+FK565%3A+a+comparative+study+with+a+CAWS-induced+model.},

doi = {10.1080/14397595.2017.1287150},

issn = {14397609},

year  = {2017},

date = {2017-11-01},

journal = {Modern Rheumatology},

volume = {27},

number = {6},

pages = {1024--1030},

publisher = {Taylor and Francis Ltd},

abstract = {Objective: Kawasaki disease (KD) occurs via activation of the innate immune system. Nucleotide oligomerization domain-1 (NOD1) is a pattern recognition receptor regulating the innate immunity. We characterized histopathology of arteritis induced by FK565, a ligand for NOD1, in mice, compared with Candida albicans water-soluble fraction (CAWS)-induced model. Methods: Vasculitis was induced by injection of FK565 or CAWS into C57BL6/J mice (n = 9 and n = 11, respectively). At 4 weeks, they were sacrificed, and plasma cytokines and chemokines were measured. Results: FK565 injection induced vasculitis mainly involving bilateral coronary arteries whereas the aortic root was diffusely affected in CAWS mice. In FK565 animals, the abdominal aorta and its branching arteries also exhibited inflammation with atherosclerosis. IL-1$alpha$, IL-1$beta$, IL-5 and RANTES were increased in FK565 group whereas IL-6, IL-13, G-CSF, IFN-$gamma$, and TNF-$alpha$ were higher in CAWS animals (p textless.05 for all variables). The total area of inflammation in FK565 mice appeared to correlate with IL-1$beta$ levels (r = 0.71},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2017c,

title = {Prognostic value of IMP3 expression as a determinant of chemosensitivity in triple-negative breast cancer},

author = {Ryuji Ohashi and Maoka Sangen and Shigeki Namimatsu and Keiko Yanagihara and Koji Yamashita and Takashi Sakatani and Hiroyuki Takei and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/28756977/},

doi = {10.1016/j.prp.2017.07.002},

issn = {16180631},

year  = {2017},

date = {2017-09-01},

journal = {Pathology Research and Practice},

volume = {213},

number = {9},

pages = {1160--1165},

publisher = {Elsevier GmbH},

abstract = {Triple negative breast cancer (TNBC) has an aggressive phenotype and poor prognosis. Neoadjuvant chemotherapy (NAC) is often used to treat TNBC, but some patients are resistant to NAC. We postulated that a subpopulation of TNBC cells expressing IMP3, an oncofetal protein, could be resistant to NAC, contributing to the poor prognosis. We investigated immunohistochemical expression of IMP3 in 42 TNBC patients who underwent NAC in association with clinical outcomes. The patients were divided into IMP3 positive (+) (n = 19) and negative (−) (n = 23) groups. High Ki67 positivity was detected in 13 patients of the IMP3 + group and 8 cases in the IMP3 – group (p = 0.03). While 9 patients in the IMP3 – group (39%) were responders, the majority of the IMP3 + patients (84.2%) were non-responders (p = 0.01). In a Cox proportional hazard model, IMP3 expression was independently associated with poor NAC response and clinical outcomes (p = 0.03 and 0.046, respectively). The IMP3 + group showed a tendency toward shorter overall survival compared to the IMP3 – group with marginal significance (p = 0.07). These findings suggest that IMP3 + tumor cells contributed to the poor clinical outcomes by exerting a chemoresistance to NAC, and that IMP3 expression has prognostic value as a biomarker for chemosensitivity and overall survival in TNBC.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2017d,

title = {Expression of MRP1 and ABCG2 is associated with adverse clinical outcomes of papillary thyroid carcinoma with a solid component},

author = {Ryuji Ohashi and Kiyoko Kawahara and Shigeki Namimatsu and Ritsuko Okamura and Takehito Igarashi and Iwao Sugitani and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/28411177/ https://pubmed.ncbi.nlm.nih.gov/28411177/?from_single_result=Expression+of+MRP1+and+ABCG2+is+associated+with+adverse+clinical+outcomes+of+the+papillary+thyroid+carcinoma+with+a+solid+component.},

doi = {10.1016/j.humpath.2017.03.012},

issn = {15328392},

year  = {2017},

date = {2017-09-01},

journal = {Human Pathology},

volume = {67},

pages = {11--17},

publisher = {W.B. Saunders},

abstract = {Solid variant of papillary thyroid carcinoma (PTC) is characterized by a solid component (SC) retaining classical cytological features of PTC. Despite some controversies, PTC with SC (PTCSC) cases have poor prognosis compared with well-differentiated PTC. We investigated if cancer stem cells (CSCs) may have a role in pathogenesis of PTCSC. PTCSC tumors (n = 27) were histologically represented by a mixture of papillary component (PC) and varying degrees of SC involving 10% to 85% of the tumor. Immunohistochemical expression of CSC markers ABCG2 and MRP1, and HBME1 and CK19 was compared between SC and PC within each tumor in association with clinicopathological parameters. ABCG2 and MRP1 were highly expressed in SC, whereas their expression was limited or absent in PC (P =.04 and.002, respectively). In contrast, expression of HBME1 and CK19 appeared higher in PC than in SC (P =.08 and.02, respectively). Higher expression of ABCG2 was associated with higher incidence of large-sized SC (P =.01). Higher expression of MRP1 was associated with higher incidence of lymphovascular invasion (P =.049). Higher expression of ABCG2 and MRP1, and lower expression of CK19 in SC were associated with higher tumor recurrence rate (P =.02,.01, and.02, respectively), and shorter disease-free survival (P textless.001 for all the variables). Our findings indicate that the tumor cells harboring CSC-like characteristics in SC could contribute to the pathogenesis of PTCSC and might account for the poor disease prognosis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2017f,

title = {Fine needle aspiration cytology of the papillary thyroid carcinoma with a solid component: A cytological and clinical correlation},

author = {Ryuji Ohashi and Yukihiro Murase and Miyuki Matsubara and Yasuhiko Watarai and Takehito Igarashi and Iwao Sugitani and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/28224716/ https://pubmed.ncbi.nlm.nih.gov/28224716/?from_single_result=Fine+needle+aspiration+cytology+of+the+papillary+thyroid+carcinoma+with+a+solid+component%3A+a+cytological+and+clinical+correlation.},

doi = {10.1002/dc.23679},

issn = {10970339},

year  = {2017},

date = {2017-05-01},

journal = {Diagnostic Cytopathology},

volume = {45},

number = {5},

pages = {391--398},

publisher = {John Wiley and Sons Inc.},

abstract = {Background: Solid variant of papillary thyroid carcinoma is a rare subtype of papillary thyroid carcinoma (PTC) containing a solid component (SC), and thus its cytological and clinicopathological features remain elusive. We examined fine needle aspiration (FNA) cytological features of PTC with variable degrees of SC (20-80% of the tumor)(PTCSC) in comparison to well-differentiated PTC (WPTC). Methods: Twenty-six cases of PTCSC were histologically stratified into major-SC (SC textgreater50% of the tumor) and minor-SC (textless49%) groups. Pre-operative FNA smears were compared between major-SC (n = 11) and minor-SC (n = 15) groups, and between PTCSC and WPTC (n = 39) groups. Results: In FNA smears of PTCSC, the presence of cohesive solid nests, trabecular patterns, overlapping, enlarged nuclei, pleomorphism, and distinct nucleolus, and the absence of colloid and psammoma bodies were noted more often than in WPTC, while classical cytological features of PTC, such as nuclear grooves and/or pseudo-nuclear inclusions, were preserved. There was no significant difference in FNA findings between the major-SC and minor-SC groups. The presence of either solid nests or trabecular patterns, and overlapping in FNA smears of PTCSC was associated with a higher recurrence rate of the tumor (P = 0.007 and P textless 0.001, respectively). Conclusion: PTCSC may pre-operatively be identified by detecting its characteristic cytological features in FNA smears, regardless of the proportion of SC within the tumor. Because clinical outcomes of PTCSC remain undetermined, it is imperative to postulate PTCSC as a differential diagnosis, even when classical nuclear features of PTC are present. Diagn. Cytopathol. 2017;45:391–398. textcopyright 2017 Wiley Periodicals, Inc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2017,

title = {Clinicopathological significance of a solid component in papillary thyroid carcinoma},

author = {Ryuji Ohashi and Kiyoko Kawahara and Shigeki Namimatsu and Takehito Igarashi and Takashi Sakatani and Iwao Sugitani and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/27882585/},

doi = {10.1111/his.13132},

issn = {13652559},

year  = {2017},

date = {2017-04-01},

journal = {Histopathology},

volume = {70},

number = {5},

pages = {775--781},

publisher = {Blackwell Publishing Ltd},

abstract = {Aims: Solid variant of papillary thyroid carcinoma (SVPTC) is characterized by a solid component (SC) involving more than 50% of the tumour with the preservation of the classical cytological features of papillary thyroid carcinoma (PTC). However, the clinical significance of SC in PTC has been rarely examined. Herein, we investigated retrospectively the clinicopathological features of PTC with various degrees (10–85%) of SC (PTCSC). Methods and results: Patients with PTCSC (n = 27) were stratified into SC-major (SC textgreater 50% of the tumour) and SC-minor (SC textless 49%) groups. The clinicopathological parameters were compared to the well-differentiated PTC (WPTC) group (n = 47). Both SC-minor (n = 18) and SC-major (n = 9) groups had increased incidence of a large-sized tumour, extracapsular extension and a high recurrence rate, compared to WPTC. Disease-free survival (DFS) of both SC-minor and SC-major was shorter than that of WPTC (P = 0.035 and P = 0.016, respectively). Overall survival was similar among all the groups. Univariate analysis revealed that SC was associated significantly with a recurrence rate (P = 0.018). Using multivariate analysis, SC appeared to be associated with a recurrence rate with borderline significance (P = 0.055). Conclusions: Our findings indicate that the presence of SC in PTC, regardless of the proportion, is associated with adverse clinical parameters and a shorter DFS.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2017b,

title = {Pleomorphic lobular carcinoma of the breast: a comparison of cytopathological features with other lobular carcinoma variants},

author = {R Ohashi and M Matsubara and Y Watarai and K Yanagihara and K Yamashita and S I Tsuchiya and H Takei and Z Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/27489086/ https://pubmed.ncbi.nlm.nih.gov/27489086/?from_single_result=Pleomorphic+lobular+carcinoma+of+the+breast%3A+a+comparison+of+cytopathological+features+with+other+lobular+carcinoma+variants.},

doi = {10.1111/cyt.12362},

issn = {13652303},

year  = {2017},

date = {2017-04-01},

journal = {Cytopathology},

volume = {28},

number = {2},

pages = {122--130},

publisher = {Blackwell Publishing Ltd},

abstract = {Objective: Pleomorphic lobular carcinoma (PLC) is a subtype of breast cancer with unique morphological features, but it remains controversial whether PLC should be considered an independent disease entity. The aim of this study was to illustrate cytopathological characteristics of PLC in comparison with other lobular carcinoma variants. Methods: We investigated clinicopathological features of PLC (n = 11) compared with those of other variants of invasive lobular carcinoma (ILC, non-PLC) (n = 32). Histological variants of the non-PLC group consisted of classic (n = 25), solid (n = 2), alveolar (n = 1) and a tubulolobular type (n = 4). A review of cytological reports and fine needle aspiration (FNA) smear samples was performed for the PLC (n = 9) and non-PLC (n = 27) groups. Results:: Patients with PLC were older, and had a higher nuclear grade and a higher incidence of axillary lymph node metastasis and triple negative phenotype than non-PLC patients (P = 0.007, P textless 0.001},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsumoto2017,

title = {Experimental Rat Skin Flap Model That Distinguishes between Venous Congestion and Arterial Ischemia: The Reverse U-Shaped Bipedicled Superficial Inferior Epigastric Artery and Venous System Flap},

author = {Noriko M Matsumoto and Masayo Aoki and Junichi Nakao and Wei Xia Peng and Yoshihiro Takami and Hiroki Umezawa and Satoshi Akaishi and Ryuji Ohashi and Zenya Naito and Rei Ogawa},

url = {https://pubmed.ncbi.nlm.nih.gov/28027237/ https://pubmed.ncbi.nlm.nih.gov/28027237/?from_single_result=Experimental+rat+skin+flap+model+that+distinguishes+between+venous+congestion+and+arterial+ischemia%3A+the+reverse+U-shaped+bipedicled+superficial+inferior+epigastric+artery+and+venous+system+flap.},

doi = {10.1097/PRS.0000000000002900},

issn = {00321052},

year  = {2017},

date = {2017-01-01},

journal = {Plastic and Reconstructive Surgery},

volume = {139},

number = {1},

pages = {79e--84e},

publisher = {Lippincott Williams and Wilkins},

abstract = {Background: The commonly used flap models have drawbacks that limit their usefulness. In the random skin flap model, flap necrosis is caused by both arterial and venous insufficiency. In the axial skin flap model, flap viability is easily affected by the pedicle blood flow and can result in complete necrosis. This study aimed to establish a new rat skin flap model that has a consistent flap survival rate and in which venous congestion and arterial ischemia can be readily distinguished macroscopically. Methods: Rats underwent reverse U-shaped bipedicled superficial epigastric artery flap elevation. The right superficial epigastric vessels formed the pedicle. In the control rats (n = 3), the left superficial epigastric vessels were left intact. In the ischemia group (n = 10), the left superficial epigastric artery was ligated. In the congestion group (n = 10), the left superficial epigastric vein was ligated. The flap was returned to the original site and sutured. The surrounding neovascularization was blocked by polyurethane film. Flap survival rates were evaluated on postoperative day 3. Results: The flaps in the ischemia and congestion groups were noticeably pale and violet, respectively. Flap necrosis was noted in the contralateral distal zone only. It started on postoperative day 2 in the ischemia and congestion groups. The mean flap survival rates of the control, ischemia, and congestion groups were 100 percent, 61.8 percent (range, 56.9 to 67.1 percent), and 42.3 percent (35.7 to 48.7 percent), respectively (all p textless 0.001). Conclusions: The flap facilitated discrimination of the effects of ischemia and congestion. This new rat skin flap model is simple and easy to construct, and has a consistent flap survival rate.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ito2017,

title = {C3 glomerulopathy and current dilemmas},

author = {Naoko Ito and Ryuji Ohashi and Michio Nagata},

url = {https://pubmed.ncbi.nlm.nih.gov/27878657/},

doi = {10.1007/s10157-016-1358-5},

issn = {14377799},

year  = {2017},

date = {2017-08-01},

journal = {Clinical and Experimental Nephrology},

volume = {21},

number = {4},

pages = {541--551},

publisher = {Springer Tokyo},

abstract = {C3 glomerulopathy (C3G) is a recently identified disease entity caused by dysregulation of the alternative complement pathway, and dense deposit disease (DDD) and C3 glomerulonephritis (C3GN) are its components. Because laboratory detection of complement dysregulation is still uncommon in practice, “dominant C3 deposition by two orders greater than that of immunoglobulins in the glomeruli by immunofluorescence”, as stated in the consensus report, defines C3G. However, this morphological definition possibly includes the cases with glomerular diseases of different mechanisms such as post-infectious glomerulonephritis. In addition, the differential diagnosis between DDD and C3GN is often difficult because the distinction between these two diseases is based solely on electron microscopic features. Recent molecular and genetic advances provide information to characterize C3G. Some C3G cases are found with genetic abnormalities in complement regulatory factors, but majority of cases seem to be associated with acquired factors that dysregulate the alternative complement pathway. Because clinical courses and prognoses among glomerular diseases with dominant C3 deposition differ, further understanding the background mechanism, particularly complement dysregulation in C3G, is needed. This may resolve current dilemmas in practice and shed light on novel targeted therapies to remedy the dysregulated alternative complement pathway in C3G.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Takata2016,

title = {Increased expression of PDIA3 and its association with cancer cell proliferation and poor prognosis in hepatocellular carcinoma},

author = {Hideyuki Takata and Mitsuhiro Kudo and Tetsushi Yamamoto and Junji Ueda and Kousuke Ishino and Wei Xia Peng and Ryuichi Wada and Nobuhiko Taniai and Hiroshi Yoshida and Eiji Uchida and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/28101228/},

doi = {10.3892/ol.2016.5304},

issn = {17921082},

year  = {2016},

date = {2016-12-01},

journal = {Oncology Letters},

volume = {12},

number = {6},

pages = {4896--4904},

publisher = {Spandidos Publications},

abstract = {The prognosis of hepatocellular carcinoma (HCC) is unfavorable following complete tumor resection. The aim of the present study was to identify a molecule able to predict HCC prognosis through comprehensive protein profiling and to elucidate its clinicopathological significance. Comprehensive protein profiling of HCC was performed by liquid chromatography–tandem mass spectrometry. Through the bioinformatic analysis of proteins expressed differentially in HCC and non–HCC tissues, protein disulfide–isomerase A3 (PDIA3) was identified as a candidate for the prediction of prognosis. PDIA3 expression was subsequently examined in 86 cases of HCC by immunostaining and associations between PDIA3 expression levels and clinicopathological characteristics were evaluated. The Ki–67 index and apoptotic cell death of carcinoma cells were examined by immunostaining and terminal deoxynucleotidyl transferase dUTP nick–end labeling assay in 24 cases. The results demonstrated that PDIA3 was expressed in all 86 HCC cases; 56 HCC cases (65%) exhibited high expression of PDIA3 and 30 (35%) exhibited low expression. The disease–free and overall survival times of HCC patients with high PDIA3 expression were significantly shorter than in HCC patients with low expression. Furthermore, increased expression of PDIA3 was associated with an elevated Ki–67 index, indicating increased cancer cell proliferation and a reduction in apoptotic cell death. Taken together, these results suggest that PDIA3 expression is associated with tumor proliferation and decreased apoptosis in HCC, and that increased expression of PDIA3 predicts poor prognosis. PDIA3 may therefore be a key molecule in the development of novel targeting therapies for patients with HCC.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Wada2016a,

title = {mRNA expression of delta-HER2 and its clinicopathological correlation in HER2-overexpressing breast cancer},

author = {Ryuichi Wada and Soroku Yagihashi and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/27840986/ https://pubmed.ncbi.nlm.nih.gov/27840986/?from_single_result=mRNA+expression+of+delta-HER2+and+its+clinicopathological+correlation+in+HER2-overexpressing+breast+cancer},

doi = {10.3892/mmr.2016.5892},

issn = {17913004},

year  = {2016},

date = {2016-12-01},

journal = {Molecular Medicine Reports},

volume = {14},

number = {6},

pages = {5104--5110},

publisher = {Spandidos Publications},

abstract = {Delta-human epidermal growth factor receptor 2 (HER2) is a splice variant of HER2, which lacks 16 amino acids in the extracellular domain. The aim of the present study was to elucidate the expression of delta-HER2 and its clinicopathological correlation in Japanese patients with HER2-overexpressing breast cancer. A total of 40 cases of HER2-overexpressing breast cancer were investigated. The mRNA expression levels of wild type (wt)-HER2 and delta-HER2 were quantitated by quantitative polymerase chain reaction using RNA extracted from formalin-fixed paraffin-embedded specimens. In addition, the activated form of phosphorylated-SRC (pSRC) and Ki-67 were detected by immunostaining. The mRNA expression levels of wt-HER2 varied largely, and the mRNA levels of delta-HER2 were correlated with those of wt-HER2. Furthermore, the enhanced immunostaining of pSRC was detected in 15 cases. Unsupervised hierarchal clustering of wt-HER2, delta-HER2 and pSRC was used to separate the cases into three clusters. In the first cluster, the levels of wt-HER2 and delta-HER2 were low, and pSRC immunostaining was low. Two other clusters were characterized by moderately and highly increased levels of wt-HER2 and delta-HER2 mRNA expression and enhanced pSRC. The delta/wt ratio was higher in the first cluster. Positive lymph node status and recurrence were more frequent in the first cluster compared with those in the two other clusters. Furthermore, the delta/wt ratio was significantly increased in lymph node-positive and recurrent cases, compared with in lymph node-negative and non-recurrent cases. The present study demonstrated that delta-HER2 was expressed in Japanese patients with HER2-overexpressing breast cancer. High mRNA expression levels of delta-HER2 were associated with pSRC and increased proliferation of tumor cells. A poor prognosis may be predicted by the increase in delta/wt ratio in HER2-overexpressing breast cancer.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2016c,

title = {Prognostic significance of mucin expression profiles in breast carcinoma with signet ring cells: A clinicopathological study},

author = {Ryuji Ohashi and Ayako Hayama and Keiko Yanagihara and Koji Yamashita and Takashi Sakatani and Hiroyuki Takei and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/27846863/ https://pubmed.ncbi.nlm.nih.gov/27846863/?from_single_result=Prognostic+significance+of+mucin+expression+profiles+in+breast+carcinoma+with+signet+ring+cells%3A+a+clinicopathological+study.},

doi = {10.1186/s13000-016-0584-1},

issn = {17461596},

year  = {2016},

date = {2016-11-01},

journal = {Diagnostic Pathology},

volume = {11},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: Signet ring cells (SRCs) often accompany gastrointestinal carcinoma, referred to as SRC carcinoma; however, breast cancers containing SRCs have not been well characterized, leaving the prognostic significance of SRCs undetermined. We have described clinicopathological characteristics of patients with breast cancer containing SRCs in relation to the expression levels of MUC1, MUC2, MUC4, MUC5AC, and MUC6. Methods: Twenty-two breast cancer cases with variable degrees of SRC population were retrospectively studied. Each case was categorized as high (textgreater31%) or low (textless30%) SRC tumor. The SRCs were morphologically classified into the intra-cytoplasmic lumen (ICL) type, or the non-ICL type. The expression levels of MUC1, MUC2, MUC4, MUC5AC and MUC6 were determined immunohistochemically. Depending on its subcellular localization, MUC1 was categorized as the luminal and cytoplasmic (LC) type, or the cytoplasmic with circumferential membranous accentuation (CM) type. These histological findings were compared with other clinicopathological parameters. Results: The series consisted of invasive ductal carcinoma (n=9), invasive lobular carcinoma (n=9), and mucinous carcinoma (n=4) cases. The SRC population accounted for 8-81% of the tumor cells. Eight cases had ICL type SRCs, and the remaining 14 had non-ICL type SRCs. Neither the high (n=12) and low (n=10) percentage of SRCs, nor the SRC types affected the clinicopathological parameters. In the low MUC1 group (n=11), larger tumors, higher nuclear grade, lymph node metastasis, and negativity for estrogen receptor was more frequently identified compared to the high MUC1 group (n=11; p=0.01},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yamamoto2016,

title = {Identification of aldolase A as a potential diagnostic biomarker for colorectal cancer based on proteomic analysis using formalin-fixed paraffin-embedded tissue},

author = {Tetsushi Yamamoto and Mitsuhiro Kudo and Wei Xia Peng and Hideyuki Takata and Hideki Takakura and Kiyoshi Teduka and Takenori Fujii and Kuniko Mitamura and Atsushi Taga and Eiji Uchida and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/27468721/},

doi = {10.1007/s13277-016-5275-8},

issn = {14230380},

year  = {2016},

date = {2016-10-01},

journal = {Tumor Biology},

volume = {37},

number = {10},

pages = {13595--13606},

publisher = {Springer Netherlands},

abstract = {Colorectal cancer (CRC) is one of the most common cancers worldwide, and many patients are already at an advanced stage when they are diagnosed. Therefore, novel biomarkers for early detection of colorectal cancer are required. In this study, we performed a global shotgun proteomic analysis using formalin-fixed and paraffin-embedded (FFPE) CRC tissue. We identified 84 candidate proteins whose expression levels were differentially expressed in cancer and non-cancer regions. A label-free semiquantitative method based on spectral counting and gene ontology (GO) analysis led to a total of 21 candidate proteins that could potentially be detected in blood. Validation studies revealed cyclophilin A, annexin A2, and aldolase A mRNA and protein expression levels were significantly higher in cancer regions than in non-cancer regions. Moreover, an in vitro study showed that secretion of aldolase A into the culture medium was clearly suppressed in CRC cells compared to normal colon epithelium. These findings suggest that decreased aldolase A in blood may be a novel biomarker for the early detection of CRC.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2016,

title = {Diagnostic value of fine needle aspiration and core needle biopsy in special types of breast cancer},

author = {Ryuji Ohashi and Miyuki Matsubara and Yasuhiko Watarai and Keiko Yanagihara and Koji Yamashita and Shin ichi Tsuchiya and Hiroyuki Takei and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/26134558/},

doi = {10.1007/s12282-015-0624-9},

issn = {18804233},

year  = {2016},

date = {2016-07-01},

journal = {Breast Cancer},

volume = {23},

number = {4},

pages = {675--683},

publisher = {Springer Tokyo},

abstract = {Background: Although fine needle aspiration (FNA) biopsy is an established tool to assess breast lesions, there has been a trend toward using core needle biopsy (CNB) instead. The aim of this study was to compare the diagnostic accuracy of FNA and CNB in special types of breast cancer. Methods: A retrospective review of diagnostic results of pre-operatively performed FNA or CNB, or a combination of the two, was conducted. The cases include histologically proven invasive ductal carcinoma of no special type (NST n = 159), invasive lobular carcinoma (ILC n = 65), mucinous carcinoma (MUC n = 51), and apocrine carcinoma (APO n = 25). Results: The absolute diagnostic sensitivity of FNA to detect malignancy in ILC and APO patients was inferior to that of NST patients (p textless 0.001 for ILC and APO). Within each cancer type, the sensitivity of CNB was higher than that of FNA in the ILC and APO patients (p textless 0.001 and p textless 0.05, respectively). As for NST and MUC patients, FNA and CNB had equivalent sensitivity. The sensitivity of FNA alone significantly improved when combined with CNB in NST, ILC and APO patients (p textless 0.05, p textless 0.001, and p textless 0.05, respectively). Conclusions: Our results suggest that FNA has less diagnostic accuracy than CNB for ILC and APO; thus, the use of CNB should be encouraged when these types of cancer are clinically suspected or when the initial FNA is inconclusive.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2016d,

title = {Mucinous carcinoma of the breast: A comparative study on cytohistological findings associated with neuroendocrine differentiation},

author = {R Ohashi and T Sakatani and M Matsubara and Y Watarai and K Yanagihara and K Yamashita and S Tsuchiya and H Takei and Z Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/26804749/},

doi = {10.1111/cyt.12298},

issn = {13652303},

year  = {2016},

date = {2016-06-01},

journal = {Cytopathology},

volume = {27},

number = {3},

pages = {193--200},

publisher = {Blackwell Publishing Ltd},

abstract = {Objective: Mucinous carcinoma (MCA) may show neuroendocrine differentiation (ND), but the cytological features characteristic of ND remains elusive. We compared fine needle aspiration (FNA) findings of MCA between cases with high and low degrees of ND. Methods: Histological sections of 37 MCA cases were immunohistochemically evaluated for expression of chromogranin A and synaptophysin, and were graded as 0 to 3+ degrees of ND. They were divided into low ND (grade 0 and 1+) and high ND (grade 2+ and 3+) groups. Pre-operative FNA samples of each group were assessed for cytological features. Results: The mean age of the high ND group (n = 18) was higher than the low ND group (n = 19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kanzaki2016,

title = {Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma},

author = {Akiko Kanzaki and Mitsuhiro Kudo and Shin Ichi Ansai and Wei Xia Peng and Kousuke Ishino and Tetsushi Yamamoto and Ryuichi Wada and Takenori Fujii and Kiyoshi Teduka and Kiyoko Kawahara and Yoko Kawamoto and Taeko Kitamura and Seiji Kawana and Hidehisa Saeki and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/26782292/},

doi = {10.3892/ijo.2016.3323},

issn = {17912423},

year  = {2016},

date = {2016-03-01},

journal = {International Journal of Oncology},

volume = {48},

number = {3},

pages = {1007--1015},

publisher = {Spandidos Publications},

abstract = {In the histopathological diagnosis of cutaneous tumors, the differential diagnosis of squamous cell carcinoma (SCC) with crateriform architecture and keratoacanthoma (KA) is often difficult so an accurate understanding of the biological features and the identification of reliable markers of SCC and KA are crucial issues. Insulin-like growth factor 2 mRNA-binding protein-3 (IGF2BP3, also known as IMP3) is thought of as a bona fide oncofetal protein, which is overexpressed and is involved in cell proliferation, migration, and invasion in several kinds of tumors. However, the role of IMP3 in cutaneous SCC and KA has not been well studied. Therefore, we focused on studying the biological functions of IMP3 in SCC and KA. In human skin SCC cell lines, HSC-1 and HSC-5, and the human keratinocyte cell line, HaCaT, IMP3 mRNA levels were significantly higher than that of normal human skin. The knockdown of IMP3 expression reduced the proliferation of HSC-1, and significantly reduced invasion by HSC-1 and HSC-5. In contrast, the knockdown of IMP3 did not significantly affect invasion by HaCaT cells. In immunohistochemical studies of SCC and KA tissues, the Ki-67 labeling index (LI) of the suprabasal cell layer was significantly higher in SCC, compared with KA tissues and the tumor-free margin (TFM) adjacent to SCC and KA. Most SCC tissues stained strongly positive for IMP3, but KA tissues and TFM were mostly negative for IMP3. The Ki-67 LI of the IMP3-positive group was significantly higher than that of the IMP3-negative group in the suprabasal cell layer of SCC. These results suggest that IMP3 plays an important role in proliferation and, more significantly, in the invasion of SCC, and may be a suitable marker for the histopathological diagnosis of SCC with a crateriform architecture and KA. Furthermore, IMP3 may potentially be a new therapeutic target for SCC.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2016b,

title = {Fine needle aspiration cytology of signet ring cells in breast carcinoma: A cytohistological study of 11 cases},

author = {Ryuji Ohashi and Ayako Hayama and Miyuki Matsubara and Yasuhiko Watarai and Takashi Sakatani and Hiroyuki Takei and Zenya Naito},

issn = {19362625},

year  = {2016},

date = {2016-01-01},

journal = {International Journal of Clinical and Experimental Pathology},

volume = {9},

number = {6},

pages = {6414--6422},

abstract = {Signet ring cell (SRC) carcinoma is a unique subtype of adenocarcinoma; however, the clinicopathological profiles of SRCs in breast carcinoma are yet to be determined. Here, we investigated cyto-histological findings of 11 breast carcinoma cases with SRCs comprising more than 20% of the tumor cells; invasive ductal carcinoma (IDC) (n=4), invasive lobular carcinoma (ILC) (n=4), pleomorphic lobular carcinoma (PLC) (n=1), and mucinous carcinoma (MC) (n=2). The mean age of the patients was 60.2 ± 13 years, and the mean tumor size was 2.0 ± 0.8 cm. Three cases of IDC, 1 PLC, and 1 of MC had grade 2 or 3 nuclear atypia, whereas 1 case of IDC, 4 of ILC, and 1 of MC had grade 1 nuclear atypia. Axillary lymph nodes were positive for metastatic carcinoma in 1 patient with IDC and 1 with ILC. In fine needle aspiration smears, intracytoplasmic lumen (ICL) type SRCs were found to be major constituents of SRCs in ILC and PLC, while non-ICL type SRCs were predominant in IDC. MC tumors had SRCs of both ICL and non-ICL types. During our follow-up period ranging from 97 to 3650 (mean 1458 ± 1055) days, all the patients were alive without recurrence of the tumor. Our results indicate that the nature of SRCs may vary with the histological subtype of breast cancer, but its presence may not indicate poor prognosis. A large study with more cases of breast cancer with SRCs for a longer period may be needed to determine the clinical significance of SRCs.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kure2016,

title = {A case of oncocytic papillary cystadenocarcinoma of the parotid gland—Pathological and molecular features of a rare tumor},

author = {Shoko Kure and Ryuichi Wada and Wei Xia Peng and Hideaki Ishii and Taeko Kitamura and Takenori Fujii and Shunta Inai and Toshitaka Nagao and Zenya Naito},

doi = {10.1016/j.ehpc.2015.10.002},

issn = {22143300},

year  = {2016},

date = {2016-09-01},

journal = {Human Pathology: Case Reports},

volume = {5},

pages = {18--22},

publisher = {Elsevier Inc},

abstract = {We present histological, immunohistochemical and molecular features of oncocytic papillary cystadenocarcinoma, a rare neoplasm of the salivary and parotid glands, in an 82-year-old Japanese man. The resected tumor was solid nodular mass with fibrous capsule. The tumor was composed of papillary proliferation of tall columnar cells with thin vascular cores. The cytoplasm of the tumor cells was granular and eosinophilic. The tumor cells showed clear positive reaction for mitochondria and androgen receptor. GCDFP15 and HER2 were negative. Electron microscopy demonstrated numerous mitochondria in the cytoplasm of the tumor cells. Ki-67 index was 30%. Most of the tumor cells were positive for TP53, and single nucleotide polymorphism was found at codon 151. The invasion into the lymphatic spaces and capsule was noted. Although recurrence and metastasis were not noted at one and a half years after the resection, the patient needs to be followed up under careful observation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tomori2016,

title = {Florid reactive periostitis in the fifth phalange of a professional boxer: A case report},

author = {Yuji Tomori and Ryuji Ohashi and Zenya Naito and Mitsuhiko Nanno and Shinro Takai},

url = {https://pubmed.ncbi.nlm.nih.gov/28002343/ https://pubmed.ncbi.nlm.nih.gov/28002343/?from_single_result=Florid+reactive+periostitis+in+the+fifth+phalange+of+a+professional+boxer：A+case+report},

doi = {10.1097/MD.0000000000005697},

issn = {15365964},

year  = {2016},

date = {2016-01-01},

journal = {Medicine (United States)},

volume = {95},

number = {51},

pages = {e5697},

publisher = {Lippincott Williams and Wilkins},

abstract = {Rationale: Florid reactive periostitis is a rare, benign bone and chondrogenic lesion that develops most frequently in the phalanges of the hands. Although the definitive cause of florid reactive periostitis is unknown, the major inciting factor is generally considered to be trauma, including repetitive minor trauma. Patient concerns: We present a case of florid reactive periostitis affecting two contiguous phalangeal bones of the left fifth phalange of a 23-year-old male professional boxer. The patient had experienced chronic pain around the metacarpophalangeal joint of the left fifth phalange when punching with the left hand; this pain was improved but not resolved after conservative treatment. Interventions: Plain radiography of the fifth phalange revealed a bulging bone lesion on the volar side of the proximal phalanx and metacarpal of the left fifth phalange. Computed tomography also showed raised bony lesions on the volar side of these bones without bony destruction or intraregional calcification. Surgery was performed and histopathology revealed that the bone specimens comprised a mixture of fibrous connective tissue and relatively immature bone covered by bland osteoblasts. Diagnoses: These findings were suggestive of a benign bone formation process, and the lesion was diagnosed as florid reactive periostitis. Outcomes: The pain and tenderness in the left fifth phalange experienced during boxing had completely resolved by 6 months postoperatively, and 12 years postoperatively the patient had full range of motion and no recurrence of pain. Lessons: Traction force, such as those associated with "leading jabs," may induce repetitive minor trauma and subsequent periosteal damage, resulting in the gradual development of bulging bone ridges on the volar surface of the proximal fifth phalange and metacarpus of the hand. Abbreviations: FRP = florid reactive periostitis},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohashi2016a,

title = {Cytological features of complex type fibroadenoma in comparison with non-complex type fibroadenoma},

author = {Ryuji Ohashi and Miyuki Matsubara and Yasuhiko Watarai and Keiko Yanagihara and Koji Yamashita and Shin ichi Tsuchiya and Hiroyuki Takei and Zenya Naito},

url = {https://pubmed.ncbi.nlm.nih.gov/26249102/},

doi = {10.1007/s12282-015-0632-9},

issn = {18804233},

year  = {2016},

date = {2016-09-01},

journal = {Breast Cancer},

volume = {23},

number = {5},

pages = {724--731},

publisher = {Springer Tokyo},

abstract = {Background: To determine the cytomorphological features of complex type fibroadenoma (CFA), we reviewed fine needle aspiration (FNA) cytology with correlation to its histopathology findings, and compared them with non-complex type fibroadenoma (NCFA). Methods: From excisional biopsy or resected specimens of fibroadenoma (FA) cases treated at our institution from 2004 to 2013, we chose 46 patients who underwent FNA before a diagnosis of FA was established. We histologically re-classified them into two groups: CFA and NCFA. FNA diagnosis was retrospectively re-evaluated from FNA reports. We further re-assessed detailed characteristics of each FNA smears to identify cytomorphological features of CFA. Results: We found that 15 cases fulfilled the diagnostic criteria of CFA, in which 7 (46.7 %) had an FNA diagnosis of “suspicious for malignancy” or “indeterminate” while only 2 NCFA cases had that of “indeterminate” (p = 0.004). FNA smears from CFA cases showed discohesiveness, enlarged nuclei, prominent nucleoli, and fewer myoepithelial cells more often than NCFA. Although no significant difference was noted in patients' age and tumor size between CFA and NCFA, 5 CFA cases (33.3 %) were accompanied by the presence of carcinoma in the same breast or the contralateral breast while no NCFA cases had carcinoma in the breast. Conclusions: FNA of CFA can lead to erroneous or indeterminate interpretation, due to proliferative and/or hyperplastic changes of ductal epithelium with or without atypia. It is important to recognize the disease entity and characteristic cytomorphological findings of CFA to reach accurate FNA diagnosis of breast lesions.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tsuchiya2016,

title = {Characteristics of the Japanese histological classification for breast cancer: correlations with imaging and cytology},

author = {Shin-ichi Tsuchiya and Rin Yamaguchi and Kyoko Tsuchiya and Ryuji Ohashi},

url = {https://pubmed.ncbi.nlm.nih.gov/23959775/ https://pubmed.ncbi.nlm.nih.gov/23959775/?from_single_result=Characteristics+of+the+Japanese+histological+classification+for+breast+cancer%3A+correlations+with+imaging+and+cytology. http://link.springer.com/10.1007/s12282-013-0488-9},

doi = {10.1007/s12282-013-0488-9},

issn = {1340-6868},

year  = {2016},

date = {2016-07-01},

journal = {Breast Cancer},

volume = {23},

number = {4},

pages = {534--539},

publisher = {Springer Tokyo},

abstract = {The Japanese histological classification of breast cancer is a unique classification that was published as the 2nd edition of the General Rules for Clinical and Pathological Recording of Breast Cancer in 1971. According to this classification, breast cancers are divided into three subgroups: noninvasive, invasive, and Paget's disease. Invasive carcinomas are further divided into two groups: invasive ductal carcinomas (IDCs) and special types. IDCs are the most common types, representing 70–80 % of all breast cancers, and most of them correspond to invasive carcinoma—not special type—according to the latest WHO classification (4th edition). In particular, IDCs are subdivided into three subgroups as follows: (1) papillotubular carcinoma, which is characterized by the projection of papillae into spaces, and includes cribriform and comedo patterns; (2) solid-tubular carcinoma, which is a solid cluster of cancer cells with expansive growths that form relatively sharp borders; and (3) scirrhous carcinoma, which grows in a scirrhous manner characterized by cancer nests or cells accompanied by marked fibrosis. The concept of subclassification originated in Japan. The Japanese histological classification has taken on important roles since its publication, in particular for comparisons between histology and imaging or cytology.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}